Determination of phenolic compounds in Pinus eldarica by HPLC

The antioxidant components have been identified in some pine species. Antioxidant properties of proantocyanidins reduce free radicals induced by DNA fragmentation and lipid proxidation and also proanthocyanidines could curb lipid peroxidation. In this study, we analyzed different parts of Pinus eldarica [bark, seed and needle] and assessed their antioxidant contents. Pine specimens were collected from four different geographic locations in Tehran. The HPLC method [UV detector, C[18] reverse phase column, 4.6 mm [25 cm, and water/ H[3]PO[4]/ methanol/ acetonitril as eluant] were employed for evaluating total polyphenols. The wavelength for detection of polyphenolic compounds was 280 nm in this study. The highest range of total polyphenols was detected in the bark of this pine, specially reported a considerable amount of tyrosol in Pinus eldarica. Tyrosol stimulated resistance to oxidative stress and also has anti aging effect. The high amount of total phenolic compounds in P. eldarica bark might be attractive for future research considering its health benefits

[Investigation of nitrate concentrations in some bottled water available in Tehran]

Bottled water is a main beverage at many developed and developing countries. It can be polluted with chemical agents. One of these agents is nitrate which affects the safety of bottled and mineral water and cause healthy effects on consumer health. Today's consumption of bottled water get a grate develop, thus infer of the quality of this matter is compulsive for each consumer. A descriptive-analytical and cross- sectional study was done with the aim of determination of nitrate in bottled water available in market of Tehran City in 2007. In this study, 18 samples of six various manufacturers of product were examined. Results showed that the mean of nitrate is 9.02 mg/L and all samples have nitrate bellow 50 mg/L and they are at the standard level. Also, the results of the examinations have good correspondence with the concentration of nitrate on bottled water labels and according to statistic meaningful relation, was considered. The nitrate content of these bottled water available in market of Tehran city is located at the level of national and global standards and it can not be a serious problem for health of consumer