[Assessment of aerobic training with selenium nanoparticles supplementation effects on cytokines levels of liver tissue in 4T1 breast cancer mice]

Introduction: The liver is known as a tissue highly sensitive to immunological changes such as cytokines activity. It has been shown that using selenium nanoparticle supplementation and exercise training separately can effectively decrease tumor volume and improve immune responses. The aim of this study was to examine the effects of aerobic interval training and supplementation of selenium nanoparticles on protein expression of cytokines in liver tissue in mice with breast cancer

Materials and Methods: Sixty-four Balbc mice were divided into eight groups. Experimental groups received aerobic interval training and selenium nanoparticles supplementation orally for 6 weeks, before and after cancer induction. At the end of the sixth week, after tumor injection, proteins levels of IL-6, IL-4, TNF-alpha and INF-gamma were measured in liver tissue by the enzyme-linked immunosorbent assay [ELISA] method

Results: Results of the current study showed that IL-6, TNF-alpha and IL-4 protein levels decreased in liver tissue of the breast cancer tumor [P<0.05]. Aerobic interval training caused significant increase in IL-6 and TNF-alpha protein levels in liver tissue [P<0.05]. Increase in INF-gamma cytokine levels in liver tissue was also observed, following selenium nanoparticle supplementation [P<0.05]. Results also showed that tumor volume decreased following exercise training and administration of selenium nanoparticles administration

Conclusion: Cytokines changes in liver tissue occurred in conjunction with decrease in tumor volume. It is possible that simultaneously using selenium nanoparticles and exercise training with immune system reinforcement could help in reducing tumor volume and modulating cytokine levels in liver

acetyl hydrolase-activity in peritoneal fluids of women with endomeiciosis

This study aimed to investigate whether platelet activating factor acetylhydrolase may play a role in the pathophysiology of endometriosis. Peritoneal fluids were obtained at laparoscopy from 16 fertile women without endometriosis and 12 women with mild endometriosis. Platelet activating factor acetylhydrolase activity was assayed in serum and peritoneal fluid by the release of 3H-acetate from 3H-platelet activating factor during a 15-minute incubation, platelet-activating factor acetylhydrolase activity [mean + st and ard error of the mean] was significantly lower [P <0.01] in peritoneal fluids from women with mild endometriosis [29.5 +/- 2.7%] than in controls [39.9 +/- 3.4%]. The platelet-activating factor acetylhydrolase-cholesterol and albumin ratios were also significantly lower in peritoneal fluids of women with endometriosis compared with controls. These data suggested that mild endometriosis is associated with decreased platelet-activating factor acetylhydrolase activity in peritoneal fluid