Prognostic value of TNF alpha mRNA and VEGF mRNA expression in patients with chronic hepatitis C geno-type-4, with and without cirrhosis and hepatocellular carcinoma to predict disease outcome

The study aimed to clarify whether vascular endothelial growth factor mRNA [VEGF mRNA] and TNF alpha mRNA in the HCC tissues on top of HCV with and without cirrhosis obtained from specimens after curative hepatic resection has a prognostic value and recurrence predictive value compared to other tumor criteria. A total of 160 patients were studied. The preoperative laboratory, radiological and staging to patients was done. Using in situ hybridization technique, VEGF mRNA and TNF alpha mRNA were determined in liver tissues of, 10 controls, 50 with HCC, 50 with HCV without cirrhosis and 50 HCV with cirrhosis. The results showed that in HCC cases there was positive correlation between increasing age, loss of weight, INR and AFP but not in other cases of CHC with or without cirrhosis. AFP, vascular invasion, encapsulation, tumor size and grade and platelet count were related to patients outcome and recurrence of tumor after follow up of most cases for 3 years. The expression of VEGF in liver tissues was proportional to progress of viral hepatitis to cirrhosis with more expression in cases progressed to malignant changes. More expression of VEGF in HCC was more evident with intense expression in cases with Vascular and capsular invasion and higher level of AFP. Expression of TNF alpha mRNA and VEGF mRNA shows increasing expression with positive correlation to progression of viral hepatitis to cirrhosis with more positive with cases developed HCC

Portal tract lymph and blood vessels changes in human chronic viral hepatitis, schistosomiasis and hepatocellular carcinoma; immunohistochemical and morphometric study

Study of morphometric changes occurring in hepatic blood and lymph vessels in different chronic liver lesions with portal hypertension including viral hepatitis [B and C], Schistosomiasis and hepatocellular carcinoma [HCC] on top of cirrhosis, and to find the relationship of these changes to liver fibrosis, portal hypertension and HCC, using a combination of immunohistochemical and computer-based methodology. After clinco-pathoilogical evaluation of human patients with chronic hepatic lesion associated with portal hypertension, the chronic vital hepatitis C [n = 19], B and C [n = 12], Schistosomiasis [n = 10] and HCC on top of cirrhosis [n = 9] and 10 non-hepatic patients as control have been studied. Morphometric assessment of the number and area of blood vessels and lymphatics as well as the percentage of hepatic fibrosis per tissue section, all were done using computer soft-ware [KS 400] on image analysis system and using a combination of immuno-histochemical computer-based methodology. Immuno-histochernical staining of blood vessels, for factor VIII and portal lymphatic for a smooth muscle actin were done using monoclonal antibodies. Hepatic fibrosis was stained by sirius-red. Portal tract area and the relative fibrosis per liver tissue section were significantly increased with increasing grade of hepatitis activity in the non malignant groups compared to the control cases, with high significance in Schistosomiasis hut in HCC insignificant fibrosis compared to other disease and to control groups. There was a significant increase in the number of blood vessels per portal tract in the disease groups, highly significant in Schistosomiasis and HCC compared to the control cases but the percent of blood vessel area was significantly reduced in HCC than Schistosomiasis. This angiogenic change was directly proportional with grades of hepatitis activity and esophageal varices as it was significant with grade III activity and histological grade of HCC. There was significant increase in the number of lymphatic vessels per portal tract in all diseased groups with highest significance in Schistosomiasis group compared to the control group. There was insignificant increase in number and significant increases of area of lymphatics in HCC. This lymphangiogenesis change was directly proportional with grades of hepatitis activity but insignificant in all groups of patients. The advancement of hepatic fibrosis and portal hypertension in chronic liver disease including Schistosomiasis and viral hepatitis [B and C] was associated with significant increase in the number of both portal blood and lymphatic vessels. There was highly significant increase in the surface area of lymphatic vessels in Schistosomiasis. There was marked angiogenesis in HCC. Further study of these factors could be a target for further work