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Journal of the Faculty of Medicine-Baghdad. 1987; 29 (2): 117-27
em Inglês | IMEMR | ID: emr-9020

RESUMO

Human and bovine oocytes were frozen slowly [0.30 °C/min and 0.27 °C/min respectively] in liquid nitrogen. The survivals of the thawed oocytes [0.45 °C/min or 100 °C/min] were examined in vitro by different tests [Morphology, Biochemical and in vitro maturation techniques]. In this study we report 84% survivals, 35% in vitro maturation [IVM] and 60% IVF of the thawed oocytes by frozen-thawed sperms. The survival rates of human and bovine oocytes were nearly similar being 90% and 86.4% respectively. The freezing technique used in this study was found to be a simple and efficient method for cryopreservation of both human and bovine oocytes. We report IVF of cryopreserved bovine oocytes by frozen-thawed sperms


Assuntos
Criopreservação , Oócitos , Bovinos
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