RESUMO
Background: there are many plant extracts which can modulate pain response. Also, studies represented modulatory effects of estradiol on pain
Objectives: the aim of the present study was to evaluate the effect of hydro alcoholic extract of Portulaca oleracea [purslane] and estradiol on pain threshold in female mice
Methods: in this experimental study, 90 female balb c mice were randomly divided into the control and experimental groups which receiving low, moderate and high doses of purslane extract, estradiol and their combination. Following intraperitoneal administration of the hormone or extract, pain threshold was measured using tail flick test, and acquired data have been analyzed using ANOVA
Results: pain threshold was significantly increased after administration of moderate and high doses of purslane extract with comparing to the control group [P<0.005 and P<0.009, respectively]. Also, the administration of moderate and high doses of estradiol caused significantly increasing of the pain threshold with comparing to the control group [P<0.009 and P<0.001 respectively]. Finally, co-administration the low doses of estradiol and purslane extract and coadministration the high doses of estradiol and purslane extract resulted increasing of the pain threshold with compare to the control group, but there was not significant changed in comparing to the groups which received low doses of estradiol and extract
Conclusion: present study showed that purslane seed extract and estradiol caused increasing of the pain threshold in the female mice. Hence, these results maybe demonstrate morphine-like effects of purslane extract and estradiol
RESUMO
Background: Glycyrrhiza glabra [Licorice] has been traditionally used as a medicinal plant in Iran for treatment of diseases such as gastric ulcer and relieve intestinal spasms
Objective: In the present study, the effect of hydro-alcoholic extract of licorice rhizome on mechanical activity of isolated colon of male rats has been studied
Methods: In this experimental study, the colon tissue of 10 adult male rats were dissected and separated into two groups: experimental and control. Each group including ten strips of tissue. The segments were connected to a force transducer longitudinally and inserted to an organ bathe contained oxygenated Tyrode solution [37 [degree]C, pH=7.4]. Their mechanical activity were recorded by power lab AD instrument in basal condition, and after administration of acetylcholine [4x10-6M] and Atropine [10-6M] in the presence and absence of licorice rhizome extract [0.036 mg/ml]. Also, the mechanical activity of control group segments were recorded at the same condition with extract solvent [ethanol %70]
Results: A significant decrease in mechanical activity was found after administration of hydroalcoholic extract of licorice compared with the control group. Also, a significant decrease of mechanical activity was seen in the presence of extract and acetylcholine compared to the control group. While the mechanical activity of tissue didn't significant changed in the presence of Atropine and extract between experimental and control groups
Conclusion: We can conclude that hydro-alcoholic extract of licorice has modifying effect on colon motility, and this activity maybe occurred independent of the cholinergic system
Assuntos
Adulto , Animais de Laboratório , Masculino , Glycyrrhiza , Fitoterapia , Extratos Vegetais , Rizoma , Neurônios Colinérgicos/efeitos dos fármacos , RatosRESUMO
Background: The licorice [Glycyrrhiza glabra] rhizome has been widely used in traditional medicine for treatment of gastrointestinal diseases
Objective: In the present study, the effect of hydro-alcoholic extract of licorice rhizome on mechanical activity of isolated ileum of male rats has been studied
Methods: Seven adult male rats were anesthetized by ethyl ether, their abdomen opened, and ileum dissected and divided into 1 cm segments
The segments were connected to a force transducer longitudinally and inserted to an organ bathe contained oxygenated Tyrode solution [37 °C, pH=7.4]]
Their mechanical activity of ileum was recorded by power lab AD instrument in basal condition, and after administration of L-NAME [10[4]M] drug in the presence and absence of licorice rhizome extract [0.0175 mg/ml]
Also the mechanical activity of control group segments were recorded at the same condition with extract solvent [ethanol %70]
Results: A significant decrease in mechanical activity of the isolated ileum occurred after administration of hydro-alcoholic extract of licorice compared to the control group, while the mechanical activity of ileum tissue not significantly changed in the coadministration of L- NAME and extract between experimental and control groups
Conclusion: We can conclude that hydro-alcoholic extract of licorice has modifying effect on ileum motility, and this activity may be occurred independently to the nitrergic system