Protective effect of two anti-hepato toxic agents: silymarin and alpha-tocopherol against the subcellular toxicity induced by the hypolipidemic agent etofibrate

Etofibrate, a nicotinoyl derivative of clofibrate which belongs to the family of fibric acids. Both nicotinic acid and clofibrate are known for their hypolipidemic action and are used for the treatment of hyperlipoproteinemia. In the present study, the effect of three concentration levels of etofibrate on four rat liver lysosomal enzymes [Acid phosphatase, beta-Galactosidase, B, N-Acetyl glucosaminidase and b-Glucuronidase] for three incubation periods [30, 60 and 120 minutes] were studied in vitro, and were then compared with their combined effects together with a single therapeutic dose of each of two anti-hepato toxic agents. Silymarin and alpha-tocopherol [vitamin E] separately on the same enzymes for the same incubation periods. The results obtained showed that the three doses of etofibrate induced a marked releasing effect on the four lysosomal enzymes irrespect of the incubation time. Meanwhile, a considerable stabilization of these enzymes was observed when each of the two single doses for alpha-tocopherol and silymarin were used separately with etofibrate at the lowest concentration level

Effect of the hypolipidemic agent gemfibrozil and its combination with two anti-hepato toxic agents silymarin and alpha-tocopherol on rat liver lysosomal enzymes in vitro

Gemfibrozil, is a potent hypolipidemic agent used for the treatment of elevated levels of plasma triglycerides and very low density lipoprotein cholesterol [VLDL]. In this study, the effect of three sole graded concentrations of gemfibrozil on four rat liver lysosomal acid hydrolases [Acid phosphatase, beta-Galactosidase, b.N. Acetyl glucosaminidase and beta-Glucuronidase] were studied for three incubation periods [30, 60 and 120 minutes] in vitro. The effect of the same concentration levels of gemfibrozil, each combined with a single therapeutic dose of two anti-hepatotoxic agents: silymarin and alpha-tocopherol on the same lysosomal enzymes for the same incubation periods were also investigated. The results of this study showed that gemfibrozil displayed a highly significant releasing effect on the four lysosomal enzymes regardless of the dose or the incubation time, and that such effect presists even in the presence of the two membrane protecting agents: silymarin and alpha tocopherol

Comparative effects of anti-inflammatory drugs and an anabolic agent on the subcellular components, lysosomal enzymes and oxygen consumption of mitochondria in rat liver

The effect of oral administration of piroxicam in dose 1.8 mg/Kg [L.D] and 5.4 mg/Kg [H.D.], pirprofen in doses 8 mg/Kg [L.D.] and 24 mg/Kg [H.D.] and primobolan in dose 1.8 mg/Kg b.wt for 8 successive weeks, on the total activities and extralysosomal release of B-galactosidase [B-GAL], N-acetyl 8-glucosaminidase [B-NAG] and alpha-galactosidase [alpha-GAL] in rat liver were examined [on 10 groups]. The collective data showed that primobolan and piroxicam by the two doses has no effect on alpha-GAL activity and the enzyme release. B-NAG and B-GAL activities were affected by the high dose of piroxicam and the combined mixture with primobolan. The total enzymatic activity was inhibited, while the extralysosomal release was significantly increased. Primobolan inhibited B-GAL activity, also the low dose of piroxicam in combined mixture suppressed the total enzymatic activity of B-NAG. In addition, the high dose of pirprofen exerted an enhancement on the total enzymatic activity and the extralysosomal release while the low dose of this drug resulted in a significant increase the total activity of B-NAG and release of B-GAL. Comparative studies of the test anti-inflammatory drugs on the O[2]-consumption of rat liver mitochondria showed that the enhancement effect on the respiration, were dose dependent

Studies on some steroidal hormones III- Evaluation of steroid hormone interaction with specific nuclear proteins in-vitro

Five steroidal hormones were used for the aim of this investigation namely: testosterone, progesterone, 17-beta-oestradiol, ethynyloestradiol [EO] and norethisterone acetate [NEA]. The effect of test hormones on specific nuclear proteins and nuclear DNA of liver cells was studied. Results were compared and analyzed statistically

Studies on some steroidal hormones: II the effect of ethinyl estradiol on the actual release of some enzymes of rat liver lysosomes in-vitro

The rate of some lysosomal enzyme release was studied under the influence of varying concentrations of ethinyl-oestradiol. The release was recorded after 3 time intervals of 30, 60 and 120 minutes. The recorded release, direct effect and the actual release were calculated. Data were compared and analysed statistically

Studies on some steroidal hormones

The release of lysosomal enzyme isolated rat liver following exposure to varying concentrations of 17 beta- oestradiol in-vitro is hereby presented. The direct effect of the hormone on the enzyme activities was determined, and the actual release of the enzymes was recorded. Differences between enzyme patterns in relation to hormonal concentration levels and time of exposure were compared