RESUMO
Background: Cytisus scoparius is an ornamental and highly aromatic plant. The petals and stamens in aromatic plants are important allergenic agents. The aim of this study is examination study of allergenicity of petal and stamen in middle-aged ontogenical stage of Cytisus scoparius L. in guinea pig
Methology: In this experimental study, 9 guinea pigs from hartly stirps were randomly selected and divided into three equal groups. Plant extracts were prepared with 16% concentration and injected intraperitoneally to guinea pigs.The first group was considered as negative control [injected with phosphate buffered saline]. The second and the third groups received petal extract and petal + stamen extract respectively. In addition intraperitoneal injections within 4 weeks, once per week, subcutaneous injection was performed in the fifth week. Finally, a week after the last injection was taken blood sampling directly from the heart of animals, and the number of eosinophils, immunoglobulin E and blood sugar levels were measured in experimental groups and analyzed data
Results: Skin test [Created wheal diameter] and serological tests [levels of immunoglobulin E, eosinophils, and blood glucose] in both groups treated with Cytisus scoparius L. significantly increased compared to controls. Electrophoretic profiles was observed about 4 protein bands [2 light protein bands and 2 pale protein bands] in the range of 27 to 85 kD in the middle-aged petal and about 6 more specific protein bands in the range of 10 to 75 kD in middle-aged petal whit stamen proteins
Conclusion: The results showed that petal of ontogenical stage of middle-aged in Cytisus scoparius have allergenicity property and and this effect is exacerbated when they are together with stamens
RESUMO
Minocycline is an antibiotic and anti-inflammatory drug. In addition, its neuroprotective effects have been shown. Since there is interaction between cell death and seizure, the aim of this study is examination of the role of minocycline on amygdala-kindled seizures in rat. In this experimental study, three groups of animals [18 rats], after stereotaxic surgery and 1-week recovery period, received twice daily kindling stimulations. In fully kindled animals of groups 1-3, minocycline was injected intraperitoneally in doses 12.5 [n = 7], 25 [n = 5] and 50 [n = 6] mg/kg, respectively, 60 minutes before stimulation. After discharge duration [ADD], stage 4 latency [S4L], Stage 5 Duration [S5D] and Seizure Duration [SD] were recorded and compared with related control groups [the same animals that had received saline 1 day before]. A p-value of less than 0.05 was considered to represent a significant difference. In fully kindled animals who had received minocycline [50 and 25 mg/kg], ADD decreased significantly. When minocycline was delivered, S5D decreased 38.3% [p < 0.001], 34% [p < 0.05], and 100% [p < 0.001] in 12.5, 25, and 50 mg/kg group animals, respectively. S4L and SD parameters respectively increased and decreased significantly only in group 50 mg/kg. According to the obtained results, it may be concluded that in fully kindled rats, application of minocycline has anticonvulsant effect on kindling model of epilepsy
RESUMO
Low-frequency stimulation [LFS] [< 5 Hz] is a suitable approach for treating seizures caused by epilepsy. Different patterns of LFS have different effects on epileptic seizures. Since no LFS pattern has so far been determined to have the best efficacy in seizure quenching, this study investigated the effect of 60-min application of LFS on amygdala-kindled seizures in rat. In this experimental study, two groups of animals [14 rats] received twice-daily kindling stimulations after stereotactic surgery and a 1-week recovery period. In group 1 [n=6], fully kindled animals received daily kindling stimulations for four days, while in group 2 [n=8], kindled animals received LFS [1 Hz, 0.1 ms pulse duration, ½ afterdischarge threshold intensity] four times [in 24-hour intervals] exactly before kindling stimulations. Afterdischarge duration [ADD], stage 2 latency [S2L], stage 4 latency [S4L], and stage 5 duration [S5D] were recorded and compared with related control groups [the same animals that had received only kindling stimulation]. Data was analyzed using repeated-measures ANOVA and post-hoc Tukey's test for within-group comparisons and Student's t-test for between-group comparisons. The first LFS application resulted in a significant increase of S2L and S4L by 91.5% [p < 0.05] and 79.5% [p < 0.01] compared with control group, respectively. Four-day consecutive LFS applications decreased S5D significantly, so that the first LFS application decreased S5D by 100% [p < 0.001]. In the first 2 days of LFS application, ADD decreased significantly by 46.9% and 40.4% [p < 0.05], respectively, relative to control. The results suggest that LFS application immediately before seizure initiation has anticonvulsant effects