RESUMO
To determine the prevalence of extended spectrum beta-lactamase among Enterobacteriaceae isolated from blood culture in a tertiary care hospital. We carried out this study at the Armed Forces Hospital, Riyadh, Kingdom of Saudi Arabia during the period between January 2003 - December 2004. We tested a total of 601 isolates of the family Enterobacteriaceae from blood culture for the prevalence of extended spectrum beta-lactamase [ESBL] production by the standardized disc diffusion method and confirmed by the ESBL E test strips. Ninety-five [15.8%] of the isolates were ESBL producers. Among these, 48.4% were Klebsiella pneumoniae [K. pneumoniae] followed by15.8% of both Escherichia coli [E. coli] and Enterobacter cloacae [Ent. cloacae]. Other isolates produced ESBL in low numbers. Klebsiella pneumoniae produced ESBL in significant numbers. Extended spectrum beta-lactamase gram-negative bacilli present significant diagnostic and therapeutic challenges to the management of infections due to these organisms. Microbiology laboratories should start reporting ESBL producing Enterobacteriaceae organism due to their importance in respect to antibiotic therapy and infection control aspects
Assuntos
Humanos , Infecção Hospitalar/tratamento farmacológico , Enterobacteriaceae/isolamento & purificação , Enterobacteriaceae/efeitos dos fármacos , Infecções por Enterobacteriaceae , Prevalência , Testes de Sensibilidade Microbiana , Resistência beta-Lactâmica , HospitaisRESUMO
Incidence of bacterial enteric pathogens among Saudi children below the age of 10 years. Fifty thousand and four hundred stool samples were retrospectively investigated for bacterial pathogens from January 1988 to December 1996. The infected children were divided into 3 age groups. All enteropathogens were identified by conventional methods, Api 20E and sero agglutination test. A total of 3123 [6%] positive stool specimens were identified during that period. Of these positives, 2791 [99%] were stool samples received from out-patients. Salmonella food poisoning species were dominant pathogenics organisms [56%] followed by Camplobacter species [28%] and Shigella species [15%]. Food born infection is common in Saudi children and is likely to be associated with food hygiene. Antimicrobial resistance was found to be common amongst the Salmonella and Shigella isolates. Treatment of infected patients if indicated, should be guided by the in-vitro susceptibility testing