Dietary Brown Propolis Extract Modulated Nonspecific Immune System and Intestinal Morphology of Pacu Piaractus mesopotamicus

Abstract The immunomodulatory and growth promoter effects of brown propolis ethanolic extract (PEE) were determined in pacu. Fish (28.4±0.4 g) were randomly distributed into 12 polyethylene circular tanks (250 L, 30 fish per tank) and fed for 60 days with a commercial diet (32% crude protein) supplemented with 0.0, 1.5, 3.0 and 4.5% (v/w) of PEE in a totally randomized experimental design (n=3). Fish organic defense cells numbers such as thrombocytes and neutrophils increased (p<0.05) in fish fed 3.0% dietary PEE. Serum lysozyme concentration also increased (p<0.05) in fish fed 1.5 and 3.0% PEE supplementation when compared to unsupplemented fish. Growth parameters were not influenced (p>0.05) by treatments. Moreover, dietary PEE decreased (p<0.05) fish intestinal muscular thickness when compared to control treatment. Intestine villi height also significantly decreased in fish fed 3.0% PEE. Long term dietary PEE at 3.0% supplementation level modulated fish nonspecific immune system and it is a non-toxic substance for pacu.

Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG

The aim of this study was to evaluate the inflammatory response kinetics after experimental inoculation with BCG in the primitive Arius sp. fish. The BCG was applied through the intramuscular injection in the caudal peduncular region, and the samples were collected for the analyses at days 1, 3, 7, 14, 21, and 33 post-injection. Acute phase inflammatory infiltrate was characterized by the predominant mononuclear cells, intersticial edema, and muscular tissue necrosis. As the inflammatory response evolved, a large number of multinuclear giant cells were formed containing the BCG. These giant cells were positive for the S100 protein at the histochemical analysis, which demonstrate the macrofage activity, confirmed by the ultra-structural analysis showing the lack of the cytoplasmic membrane enveloping the many nuclei within the giant cell. These results led to the conclusion that Arius sp. fish injected with the BCG showed a difuse inflammatory response characterized by a large number of mononuclear cells, absence of granuloma formation, and predominant giant cells.

Avaliou-se a cinética da resposta inflamatória induzida experimentalmente com BCG em peixes primitivos pertencentes ao gênero Arius. Os animais foram inoculados com BCG por via intramuscular na região do pedúnculo caudal, sendo realizada a coleta do material nos tempos experimentais de 1, 3, 7, 14, 21 e 33 dias pós-inoculação. A fase aguda da resposta inflamatória se mostrou na forma de infiltrado inflamatório composto predominantemente por células mononucleares, edema intersticial e necrose de tecido muscular. À medida que o processo se desenvolveu, houve formação e aumento no número de células gigantes multinucleadas envolvendo o inóculo. Essas células gigantes, ao exame imunohistoquímico, apresentaram positividade à proteína S100 indicando ação de células macrofágicas, além da ultraestrutura apontar a ausência de membrana citoplasmática entre os inúmeros núcleos presentes nas células. Em vista dos resultados obtidos podemos concluir que em peixes pertencentes ao gênero Arius sp. inoculados com BCG, verificou-se durante todo tempo experimental uma resposta inflamatória difusa composta predominantemente por células mononucleares, não havendo a formação granuloma, porém havendo o predomínio de células gigantes.

Avaliação histopatológica, imuno-histoquímica e ultraestrutural da resposta inflamatória crônica do robalo (Centropomus spp. ) ao bcg / Histopathological, immunohistochemical, and ultra-structural evaluation of chronic inflammatory response of robalo (Centropomus spp) to bcg

Objetivo do estudo foi avaliar a cinética da resposta inflamatória induzida experimentalmente com BCG em peixes modernos pertencentes ao gênero Centropomus sp. Os animais foram experimentalmente inoculados com BCG por via intramuscular na região do pedúnculo caudal sendo realizada a coleta do material nos tempos experimentais de 1, 3, 7, 14, 21 e 33 dias pós-inoculação. Afase aguda da resposta inflamatória se mostrou na forma de infiltrado composto predominantemente por células mononucleares, edemaintersticial e necrose de tecido muscular. À medida que o processo se desenvolve, observa-se a organização do foco lesional na forma de um granuloma formado por células epitelióides. Essas células, ao exame imunohistoquímico apresentaram positividade a proteína S100 e citoqueratina, indicando características macrofágicas e secretória, além de apresentarem ao exame ultra-estrutural a presença de desmossomos entre as células adjacentes. Não houve participação efetiva de células gigantes e pigmentares, sugerindo ser uma característica relacionada à espécie.

This study set out to evaluate inflammatory response kinetics afterexperimental inoculation with BCG in modern Centropomus sp. fish.BCG was applied through intramuscular injection in the caudalpeduncular region, and samples were collected for analyses at days 1,3, 7, 14, 21, and 33 post-injection. Acute phase inflammatory infiltratewas characterized by predominant mononuclear cells, intersticial edema,and muscular tissue necrosis. As the inflammatory response evolved,the lesion organized into a granuloma formed by epithelioid cells.These epithelioid cells were positive for the S100 protein and cytokeratinat histochemical analysis, which demonstrates macrophage andsecretory activity, besides ultra-structural analysis showed desmosomicjunctions within adjacent cells. It didn’t have an expressive participationof giant cells and pigment containing cells in the inflammatory reaction,suggesting that it’s a specie-specific characteristic.