RESUMO
The immune response against ILT vaccination using different routes [intraocular, spray, dipping and drinking water] was evaluated. The criteria of evaluation depended on quantitative agar gel precipitation test [QAGPT], counter immunoelectrophoresis [QCIE], lymphocyte transformation [LT] test and protection against challenge of vaccinated and control groups. The results indicated no significant difference between different vaccinated groups either by QAGPT or QCIE, while revealed significant difference between different vaccinated groups on the following descending order: Intraocular, spray, drinking water and dipping route. Results of challenge revealed maximum protection [90%] in ocular instillation and spray procedures [in spite of adverse post-vaccination reaction was recorded in spray vaccination] followed by drinking water route [80%], while dipping and non-vaccinated groups gave 40% and 20%, respectively