In vitro differentiation and culture of rauwolfia root

Stem callus of Rauwolfia serpentina was induced to differentiate roots in vitro by the supplementation of culture medium with 0.1 mg/I NAA and 0.1 mg/I BAP. Differentiated roots were excised and cultured successfully. The average increase in number of lateral roots formation was observed as 110.86% in one week. Ajmaline was the major alkaloid found in differentiated roots