RESUMO
Objective To analyze and compare the Eucommia ulmoides leaves from different producing areas by IR;To establish a TLC method for identification of Eucommia ulmoides leaves;To provide a reference for its quality control. Methods The characteristic absorption peaks were identified and the positions and intensities of Eucommia ulmoides leaves from 6 producing areas were compared by IR and second derivative spectrum, including clustering analysis; Separation and identification of the active components in Eucommia ulmoides leaves were conducted by TLC. Results There a difference exited in the characteristic absorption peaks in peakshapes, positions and intensities. And the samples from 6 producing areas could be divided into one category. Results found 6 batches of sample spots of consistent by TLC, but between samples of different producing areas trace components spots existed differences. Conclusion The two methods are direct, simple, fast and convenient, which can provide a reliable evidence for identification and quality control of the Eucommia ulmoides leaves.