RESUMO
Abstract The present study has been carried out with the seed extracts of Nyctanthes arbor-tristis L. (Parijat) and evaluates its antioxidant potential and profiling the phytochemical constituents by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The antioxidant potential of the seed extracts was measured by four different in vitro assay like 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, superoxide anion free radical scavenging activity, ferric reducing antioxidant power (FRAP) and lipid peroxidation inhibition potential (LPIP) assay. The total phenol content (TPC) and total flavonoid content (TFC) were estimated. The ethyl acetate extract (EAE) of seeds showed potential DPPH free radical scavenging activity (EC50 129.49±3.55µg/ml), superoxide anion radical (EC50 969.94±8.03µg/ml) and LPIP (EC50 452.43±5.07 µg/ml) activities. The total phenol content was maximum in aqueous extract (AQE) which was 201.00±0.20 µg/mg gallic acid equivalent. The EAE was rich with total flavonoid and it was found to be 34.50±0.40 µg/mg rutin equivalent. The EAE was subjected for phytochemical-profiling using GC-MS system. The presence of different phytoconstituents supports the medicinal value of the seeds. The results suggest that EAE constitutes a promising new source of novel compounds. Further, it can be used for isolation and purification of specific compounds which have good antioxidant activities and possess useful biological activities.
RESUMO
Objective: The objective of this study was to compare the sorption and solubility of heat‑cure and self‑cure acrylic resins in different solutions. Materials and Methods: One heat‑cure acrylic resin (Trevalon) and one self‑cure acrylic resin (Rapid Repair) were studied. Five groups of square‑shaped specimens (20 mm × 20 mm × 2 mm) were prepared for each acrylic resin and then immersed in five solutions: distilled water, artificial saliva, denture cleansing solution, distilled water, and denture cleaning solution for 12 h alternatively, artificial saliva and denture cleaning solution for 12 h alternatively at 37 ± 2°C, and tested sorption and solubility by weight gain/loss method, respectively, after 1, 6, and 11 weeks. The data were analyzed by one‑way analysis of variance followed by post hoc Tukey’s test. Results: Water sorption mean values varied from 17.5 ± 0.88 to 27.25 ± 1.04 μg/mm3 for heat cure and from 12.75 ± 0.55 to 19.75 ± 1.04 μg/mm3 for self‑cure in the different solutions after different interval periods of 1, 6, and 11 weeks. These values were statistically significant (P < 0.001). Water solubility mean values varied from 0.25 ± 0.55 to 1.5 ± 0.55 μg/mm3 for heat cure and from 1.5 ± 0.55 to 6.5 ± 0.55 μg/mm3 for self‑cure in the different solutions after different interval periods of 1, 6, and 11 weeks. These values were statistically not significant (P > 0.05). There was no linear correlation between sorption and solubility values. Overall, analysis of results showed the maximum sorption value in denture cleansing solution followed by alternative soaking in distilled water and artificial saliva. Least sorption was observed with artificial saliva followed by distilled water. Conclusion: Both heat‑cure and self‑cure acrylic resins showed varying water sorption and solubility. The results of both water sorption and solubility showed compliance with the International Standards Organization specification. No correlation was found between water sorption and solubility. Artificial saliva solution is a better storage medium than distilled water and denture cleansing solution for both heat‑cure and self‑cure acrylic resins.