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Objective@#To investigate the clinical pathological features of Barrett′s esophagus in China, and to study the relationship between the number of goblet cells and the severity of Barrett′s esophageal dysplasia.@*Methods@#From January 2008 to October 2018, in the Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, the clinical and pathological data of 453 patients who underwent gastroscopy and pathologically diagnosed with Barrett′s esophagus were retrospectively analyzed. The clinical pathological features were compared between patients with goblet cells and patients without goblet cells. Periodic acid Schiff reaction (PAS) staining was performed on pathological slides of Barrett′s esophagus with goblet cells, and the relationship between the number of goblet cells, the number of positive crypts of goblet cells and the severity of Barrett′s esophageal dysplasia was analyzed. T test and chi-square test were performed for statistical analysis.@*Results@#Among 453 patients with Barrett′s esophagus, 251 (55.4%) were males and 202 (44.6%) were females. There were 218 Barrett′s esophagus with goblet cells, including 128 males (58.7%) and 90 females (41.3%). The average onset age was (60.6±11.9) years old, and the peak onset age was between 60 and 69 years old. The appearance under endoscopy mainly was circumferential type (58.2%, 127/218). There were 235 Barrett′s esophagus without goblet cells, 123 males (52.3%) and 112 females (47.7%). The average onset age was (56.1±14.4) years old, and the peak onset age was between 50 and 59 years old. The appearance under endoscopy was mainly circumferential type (40.0%, 94/235). The incidence of dysplasia in Barrett′s esophagus with goblet cells was higher than that without goblet cells (75.7%, 165/218 vs. 37.0%, 87/235), and the difference was statistically significant (χ2=68.501, P<0.01). PAS staining showed that goblet cells were stained purplish red. The number of goblet cells, total number of crypts, the number of positive crypts of goblet cells and the proportion of positive crypts of goblet cells of Barrett′s esophagus with mild dysplasia were all significantly higher than those of Barrett′s esophagus with moderate dysplasia (95.50±40.56 vs. 40.00±13.34, 21.00±8.31 vs. 11.83±2.92, 16.50±6.17 vs. 7.50±2.47 and 0.79±0.42 vs. 0.63±0.12, respectively), and the differences were statistically significant(t=-4.503, -3.605, -4.690 and -4.340, all P<0.01).@*Conclusion@#Barrett′s esophageal dysplasia may be related with appearance of goblet cells, and the decrease or disappearance of goblet cells may indicate the progression of Barrett′s esophagus.
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Objective To investigate the clinical pathological features of Barrett's esophagus in China , and to study the relationship between the number of goblet cells and the severity of Barrett 's esophageal dysplasia.Methods From January 2008 to October 2018, in the Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital , the clinical and pathological data of 453 patients who underwent gastroscopy and pathologically diagnosed with Barrett 's esophagus were retrospectively analyzed .The clinical pathological features were compared between patients with goblet cells and patients without goblet cells . Periodic acid Schiff reaction (PAS) staining was performed on pathological slides of Barrett's esophagus with goblet cells, and the relationship between the number of goblet cells , the number of positive crypts of goblet cells and the severity of Barrett's esophageal dysplasia was analyzed .T test and chi-square test were performed for statistical analysis.Results Among 453 patients with Barrett's esophagus, 251 (55.4%) were males and 202 (44.6%) were females.There were 218 Barrett's esophagus with goblet cells , including 128 males (58.7%) and 90 females (41.3%).The average onset age was (60.6 ±11.9) years old, and the peak onset age was between 60 and 69 years old.The appearance under endoscopy mainly was circumferential type (58.2%, 127/218).There were 235 Barrett's esophagus without goblet cells , 123 males (52.3%) and 112 females (47.7%).The average onset age was (56.1 ±14.4) years old, and the peak onset age was between 50 and 59 years old.The appearance under endoscopy was mainly circumferential type (40.0%, 94/235).The incidence of dysplasia in Barrett's esophagus with goblet cells was higher than that without goblet cells (75.7%, 165/218 vs.37.0%, 87/235), and the difference was statistically significant (χ2 ＝68.501, P?0.01).PAS staining showed that goblet cells were stained purplish red .The number of goblet cells , total number of crypts , the number of positive crypts of goblet cells and the proportion of positive crypts of goblet cells of Barrett 's esophagus with mild dysplasia were all significantly higher than those of Barrett 's esophagus with moderate dysplasia (95.50 ±40.56 vs.40.00 ±13.34, 21.00 ±8.31 vs.11.83 ±2.92, 16.50 ±6.17 vs.7.50 ± 2.47 and 0.79 ±0.42 vs.0.63 ±0.12, respectively), and the differences were statistically significant ( t＝-4.503,-3.605,-4.690 and -4.340, all P?0.01).Conclusion Barrett's esophageal dysplasia may be related with appearance of goblet cells , and the decrease or disappearance of goblet cells may indicate the progression of Barrett's esophagus.
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Objective To investigate the effect of small interfering RNA (siRNA) mediated silencing of △Np73 on 5-FU chemotherapy sensitivity in SW620 colocancer cells and provide new treatment approach for the colon cancer.Methods siRNAs were transfected into SW620 colon cancer cells.The expression of △Np73 was observed.Cell viability of colon cancer cells were measured by MTr assay and cell apoptosis was assessed with flow cytometry after treatment of control siRNA or △Np73 siRNA or combined with 5-FU,respectively.The tumorigenesis was assessed by injecting △Np73 siRNA or control siRNA transfeeted SW620 colon cancer cells into nude mice,followed by treatment with 5-FU in the tumors.Results △Np73 siRNA was able to strongly inhibit △Np73 expression,however,it did not inhibit the growth of cells.Combination treatment with △Np73 siRNA and 5-FU produced significant higher apoptotic cell(42.9%) as compared with those with 5-FU treatment(18.9%) alone or those with △Np73 siRNA(8.8%) alone.The treatment with 5-FU in the xenngrafts derived from △Np73 siRNA transfected SW620 cells in nude mice can inhibitor tumor growth significantly (t=15.32,P<0.05).Conclusion △Np73siRNAs can specifically repress the expression of △Np73.Thus it sensitizess the cells to 5-FU chemotherapy in colon cancer.