RESUMO
Adenoviruses are double-stranded DNA and non-enveloped viruses. It causes different diseases in human through respiratory, urinary and gastrointestinal tracts. Interestingly, more than fifty serologic types of human adenovirus were distinguished and just serotypes 40 and 41 cause gastroenteritis in human. The purpose of this study was to evaluate the prevalence of adenovirus infection in patients with acute gastroenteritis in the population of Tehran. Between May 2008 and May 2009, 293 stool samples were collected from patients with acute gastroenteritis. After DNA extraction, PCR procedures were performed, using designed primers. Positive adenoviruses samples were analyzed by the software and their serotypes were determined. 6 positive samples of 293 patients recruited to the hospital were children less than 5 years old. Additionally, they were diagnosed as serotype 41 of adenoviruses F group. According to our results, serotype 41 of adenovirus was an important cause of gastroenteritis in less than 5 years old children in population of Tehran
Assuntos
Humanos , Feminino , Masculino , Prevalência , Gastroenterite/virologia , Gastroenterite/microbiologiaRESUMO
Since data about prevalence of JC virus in Iranian population is scarce, this study was designed to evaluate the prevalence of JC virus in healthy individuals who had attended Fajr hospital and Farjam clinical laboratory in Tehran, Iran. JC virus is the causative agent of progressive multifocal encephalopathy [PML] in individuals with suppressed immune system. There are some evidences that this virus is responsible for some forms of cancers for example colorectal and gastric cancers in humans. Urine samples from 133 healthy individuals older than 18 years old were collected and after extraction of viral DNA, PCR was performed to determine the presence of virus. Results of the test and demographic data of subjects were entered into SPSS program and were analyzed by it. 71 subjects were male and 62 individuals were female. Mean age of the population was 42.23 +/- 13.47. From the total number of 133 subjects, 51 [38.3%] individuals were positive for the presence of JC virus. Gender had statistically significant relationship with JC virus presence [p= 0.042]. Age was not significantly related to JC virus presence status [p= 0.3]. Obtained rate of JC prevalence in this study is similar to the results of studies in India and Philippine. Because of this virus's role in AIDS and the role of this virus in gastrointestinal cancers have been revealed in recent years, the more extended studies on the prevalence of this virus in different populations in Iran is necessary
RESUMO
Familial Mediterranean fever [FMF] is an autosomal recessive disorder characterized by recurrent attacks of fever and inflammation in the peritoneum, synovium, or pleura, accompanied by pain. In this study, we examined all 10 exons to determine the most common mutations in MEFV gene as a single gene associated with FMF. In this basic study, 51 clinically diagnosed Iranian FMF patients referred to Taleghani hospital were studied. Peripheral blood was gained from them and genomic DNA was extracted according to phenol chloroform standard protocol. They were screened for the MEFV mutation using bidirectional sequencing and finally, the sequences were analyzed by related soft wares. Of 51 patients suspected to FMF, 24 [47.05%] were positive for mutation and 27 [52.95%] had no mutations. 14 patients had M694V mutation in exon10 including 4 homozygote mutation, 8 heterozygote and 4 compound heterozygote. Moreover, we could find 6 patients with M680I mutation and 2 individual [8.3%] with V721I mutation in exon 10. Only one person carried E148Q heterozygote mutation in exon 2. Our finding were compatible with others investigation that M694V mutation is the most common mutation in different populations