RESUMO
Background: HCV is the foremost cause of liver cirrhosis and hepatocellular carcinoma and its prevalence is increasing in developing countries like Pakistan. Present study is focusing on its frequency in different districts of the Punjab of Pakistan
Setting: Different districts of Punjab
Period: Jan 2010 to Dec 2010
Material and methods: 5ml venous blood was collected from each donor by using disposable syringes. Sample is transferred to vials containing anticoagulant, centrifuged and plasma was separated for further analysis.140 ul plasma of every patient was analyzed for HCV RNA Virus by Real time PCR using Artus HCV Quantification kit [Germany].For statistical analysis SPSS 16 was used
Results: A total count of 3262 samples was collected from 32 districts of the Punjab and all these samples were both rapid HCV screening and Anti HCV by Elisa positive. Out of which 2041 [62.57%] patients were detected positive for HCV, 1221 [37.4%] were not detected.49.5%patients were male while50.5% were female. 30.99% males were detected positive including patients with low viral load. 31.58% were detected positive for HCV including low positive female patients.30.71% females were detected positive for HCV, 37.5% were not detected for HCV
Conclusion: Highest prevalence of HCV among different age groups is found in age group of 36-40 years with 12.23% and lowest is found in age group > 15 years with 0.31% [p=0.009]
RESUMO
To determine the existence of autosomal recessive deafness loci in different ethnic tribes of the Punjab. Descriptive observational study. Department of Human Genetics and Centre of Excellence in Molecular Biology, University of Health Sciences, Lahore, from July 2009 to March 2012. Healthy willing subjects with autosomal recessive deafness loci were studied for selected deafness loci. Those who were unhealthy and gave history of infectious disease were excluded. DNA extraction was carried out using the inorganic method. Fluorescently labeled microsatellite markers were used for amplification of desired regions by PCR [Polymerase Chain Reaction]. Automated allele assignment was performed using the ABI PRISM GeneScan Analysis Software Version 3.7 for Windows NT Platform. Two-point LOD scores were calculated using the FASTLINK computer package [Schaffer 1996] and MLINK was used for calculation and 95% CI [confidence intervals] were calculated. One hundred and thirty two individuals of 8 families were analyzed. Three families [SAPun-03, SAPun-10 and SAPun-15] were found linked to DFNB12; two families [SAPun-05 and SAPun-17] were found linked to DFNB8/10, while three families [SAPun-06, SAPun-13 and SAPun-19] were found linked to DFNB29, DFNB36 and DFNB37 respectively. The genotyping results revealed that DFNB12 locus was the most common followed by DFNB8/10 locus, while the Loci DFNB29, DFNB36 and DFNB37 were less common