Study of trichomoniasis among egyptian male patients

In the present study, Trichomonas vaginalis was diagnosed in 28.8% male patients with urethral discharge and in 8.2% suffering from impotence and infertility. Diagnosis was based on examination of urethral discharge, urine, semen and prostatic massage fluid by wet mount, stained films and culture inoculation. Diamond's culture proved to be the method of choice

immunological aspects of cutaneous leishmaniasis in Egypt

In patients with cutaneous leishmaniasis, B and T-lymphocytes subpopulations [CD3[+], CD4[+] and CDB[+] were estimated. In cases and with different clinical forms diagnosis was made by stained smears, culture on Schneider's medium with 25% foetal calf serum. The results revealed that CD4[+] were significantly increased in early infection [less than 6 weeks]. No significant changes were found in single lesion in contrast with multiple lesions [more than 6 weeks] where CD4[+] significantly decreased than controls. CD8[+] were increased with the both the number and duration of the lesions. Follow up of cases till spontaneous clinical and parasitological cure showed no significant difference in comparison to controls. It was concluded that T-lymphocyte subpopulations through their cytokine mediators may play an important role in the mechanism of healing in cutaneous leishmaniasis

use of three immunologic tests in detection of antibodies against trichinella spiralis

Human sera [330 from patients with manifestations suggestive trichinosis] were examined for antibodies. The immunologic tests used were counter current immunoelectrophoresis [CEP], enzyme linked immunosorbent assay [ELISA] and immunoflourescence [IF]. Seropositive reactions were demonstrated in 2.7%, 5.4% and 2.4% of the sera by the 3 tests respectively. However, 2.4% of the sera were seropositive by the three tests. It seems that the use of two of these tests is valuable in diagnosis when muscle biopsy is unproductive

Comparison of three immunodiagnostic tests in detection of trichinosis in naturally infected rats

The counter current immunoelectrophoresis [CEP], the enzyme linked immunosorbent assay [ELISA] and the indirect immunofluorescent [IF] were evaluated in detection of antibodies in naturally infected rats. The test subjects included 10 infected rats as indicated by digestion method of muscle biopsy and 13 rats free from trichinosis. Of the immunodiagnostic tests evaluated, the CEP gave the highest diagnostic accuracy. The ELISA was the least accurate. The high sensitivity and specificity of the CEP for detection of anti-trichinella antibodies suggested that it could be developed as a serodiagnostic test for trichinosis