Glycation inhibition activity of 5-Sulfosalicyclic acid in diabetic condition

To study the glycation inhibition effect of Sulfosalicyclic acid. The study was conducted by using plasma from apparently healthy non diabetic and diabetic persons. Samples of different concentrations of glucose and inhibitor were incubated for 5 weeks at 37°C temperature. Glucose was estimated by glucose oxidase kit method before and after dialysis. Glycation level was assessed and measured by TBAand periodate assays. Increase in glycation was observed from 1[st] to 3[rd] week of incubation while it was decreased after 5[th] week due to formation of advanced glycation end products. Three different concentrations of inhibitor showed variable responses. It was also noted that 10 mM concentration of Sulfosalicyclic acid exhibited fairly good response to decrease glycation. Highest concentration of Sulfosalicyclic acid produced overall high level of inhibition. Periodate borohydride proved to be more reliable and sensitive glycation assay when compared to TBA test

The role of persistent anticardiolipin antibody as risk factor of ischemic stroke.

AIM: To determine the role of persistent ACA and hyperviscosity as risk factor of ischemic stroke. Methods: A study was conducted on 76 subjects whose age 40 to 70 years. Subjects consisted of 38 patients of post ischemic stroke and 38 controls with diagnosis other than stroke. Fresh blood samples were taken and mixed with EDTA for viscosity examination and serum for ACA IgM and IgG examination. The laboratory examination for persistent ACA IgM and IgG used ELISA method, while viscosity analysis was using viscometer. Statistic analysis used chi-square and multivariate analysis with logistic regression. RESULTS: In this study we found persistent ACA IgG in 25% of case group , and 2.63% in control group. Multivariate analysis showed persistent ACA IgG as risk factor for ischemic stroke with p < 0.05 and OR 14.11 (CI 95%:1.64;121.11). We found persistent ACA IgM in 2.78% of case group and 5.26% in control group. High blood viscosity was found in 15.79% case group and 10.53% in a control group. Statistical analysis showed no significant difference of viscosity (p = 0.740) and persistent ACA IgM (p = 1.000) between case and control group. CONCLUSION: study showed that persistent ACA IgG in stroke ischemic was higher than in control subjects. Blood viscosity examination and persistent ACA IgM did not show significant difference. While persistent ACA IgG with OR 14.11 (CI: 1.64; 121.11) was the risk factor for ischemic stroke. Blood viscosity and persistent ACA IgM were not risk factors for ischemic stroke.

The efficacy of specific patterns of movements and brain exercises on the cognitive performance of healthy senior citizen in Jakarta.

The objective of this study was to determine the efficacy of brain movement and exercise in healthy senior subjects. Brain movement and exercise or Gerak dan Latih Otak (GLO) which comprised of muscles stretching, breathing exercise, and crossing the body’s midline of the eyes, head and extremities were performed two times weekly for two months by 70 normal healthy senior subjects. A pre- and posttest on 5 cognitive performance, consisted of visual scanning and tracking (VST), delayed recall (DR), verbal fluency (VF), digits backward (DB), and trail making test-B (TMT-B). The age range of the subjects was 48 to 70 years and duration of education ranged from 6 to 18 years or more. The results of the statistical analyses showed that all the subjects showed high significant increase performance on the five cognitive domains. Women performed significantly in all the tests, while in men the significant increase was on VST, DR, and TMT-B. No different significant increase cognitive performance could be obtained between the two age groups (below 60 years and 60 years upward) and level of education, except for the high education group. It is suggested that further studies with larger scale subjects and same methodology should be performed in several places in Indonesia.