Influence of abiotic environmental factors on the main constituents of the volatile oils of Tithonia diversifolia

ABSTRACT Tithonia diversifolia (Hemsl.) A. Gray, Asteraceae, commonly known as Mexican sunflower, is a wide distributed invasive species encountered around the world. We proposed herein to establish the relationship between different abiotic environmental factors and the variation in the production of volatile compounds in T. diversifolia, during a period of one year. Samples of leaf and inflorescence volatile oils obtained from individuals located at two different regions of Brazil were analyzed by GC-MS and the data were submitted to chemometric analysis. Based on the main constituents, the analysis allowed us to classify the volatile oils into two chemotypes, according to their geographical origin. The influence of soil nutrients, mainly Ca and P, was also observed in the composition of the volatile oils. Climate also seems to affect the constituents of the volatile oils, mainly the contents of leaf sesquiterpenes of individuals growing in areas with higher average temperatures and solar radiation levels. We can therefore highlight that the appropriate multivariate statistical analysis allowed us to propose for the first time the existence of chemotypes for the volatile oils of T. diversifolia, as well as reporting the main abiotic environmental factors related to the accumulation of the discriminant compounds in these oils.

Influence of environmental factors on the concentration of phenolic compounds in leaves of Lafoensia pacari

Lafoensia pacari A. St.-Hil., Lythraceae, a plant from the Cerrado known as pacari or dedaleiro, is widely used as an antipyretic, wound healing, anti-inflammatory, antidiarrheal and in the treatment of gastritis and cancer. Notable among the metabolite groups identified in leaves of L. pacari are the polyphenols, such as tannins and flavonoids, related to the pharmacological activities of pacari. Studies on the influence of environmental factors over production of major groups of secondary metabolites in pacari are important because they contribute data for its cultivation and harvest, and establish quantitative parameters of secondary metabolites in the plant drug. The objective of this study was to evaluate the influence of environmental factors on concentrations of phenolic metabolites in the leaves of L. pacari. Compounds quantified in the leaves were: total phenols, tannins by protein precipitation, hydrolysable tannins, total flavonoids, ellagic acid and mineral nutrients, while soil fertility was also analyzed, all over a period of one year. The data were analyzed using multivariate analysis, and the results suggest that metabolite concentrations in the leaves of this plant are influenced by seasonal factors, in particular the temperature and foliar micronutrients (Cu, Fe, Mn, Zn).