The associated microflora to the larvae of human bot fly Dermatobia hominis L. Jr. (Diptera: Cuterebridae) and its furuncular lesions in cattle

The microflora associated to furuncular lesions, larvae and pupae of Dermatobia hominis, as well as the relationships between parasite, host and microflora associated, as a comprehensive microsystem, has been studied. One hundred and two furuncular myiasis due to D. hominis larvae in several breeds of cattle were studied and the following bacterial species were significant: Staphylococcus aureus, S. epidermidis, S. warneri, Bacillus subtilis and Escherichia coli. Closely related, the microflora associated to 141 samples from first, second, third instar larva and both external surface and larval cavities has been studied. The representative associated microflora to the larvae were: S. aureus, B. subtilis, S. hycus and Moraxella phenylpiruvica, Moerella wisconsiensis, Proteus mirabilis and P. vulgaris, M. phenylpiruvica, M. wisconsiensis, P. mirabilis and P. rettgeri were the representative microflora associated to 64 pupae of D. hominis.

Primera descripcion de un brote de gastroenteritis hemorragica aguda canina en Costa Rica. / First description of an acute hemorrhagic canine gastroenteritis outbreak in Costa Rica

Es la primera descripcion de un brote de gastroenteritis hemorragica aguda canina en Costa Rica. Se estudiaron doce perros con diarrea y vomitos, se les tomaron muestras de heces y de tejidos, destinadas tanto para microscopia de luz, como para microscopia electronica de barrido y de transmision. Macroscopica y microscopicamente se observaron grandes areas congestivas y hemoragicas en duodeno y yeyuno.Al microscopio de luz se observaron una marcada perdida y descamacion del epitelio de las vellosidades y dilatacion de las criptas. Con microscopia electronica de transmision y de barrido se observo una acentuada perdida de las microvellosidades,capilares con endotelio destruido, alteraciones en el microcaliz y en las mitocondrias. El analisis de las heces por microscopia electronica de transmision con tincion negativa, revelo la presencia de particulas virales semejantes a parvovirus, lo que confirma el diagnostico de gastroenteritis hemorragica aguda canina