Detection and Typing of Human Papillomavirus in Anal Condyloma Acuminatum of HIV-positive Patients

PURPOSE: Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (+) Korean anal condyloma using PCR-based DNA microarray. METHODS: DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (+) patients (M:F=15:2, mean age 35 years, 22~59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip. RESULTS: Fourteen patients (9 HIV (+), 5 HIV (-)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (+) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (-) patients, 5 patients (55.6%) proved to have multiple infections in HIV (+) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (+) patients (44.5%) showed co-infection with high-risk HPV. CONCLUSION: Multiple infection and co-infection with high-risk types are more prevalent in HIV (+) condyloma patients compared to HIV (-) patients. HPV types on HIV (+) male anal condyloma are similar to those detected in the Korean female uterine cervix.

A Case Study of Constructing a Medical Web-Casting System / 대한의료정보학회지

As digital technologies develop further, computer and communication networks combined more and more. In addition,the integrated systems of broadcasting and communication accelerate the disappearance of business borderline between them.Intemet broadcasting technology such as Video on Demand(VOD) system is a good example. There are also digitized, two-way communication systems such as web-casting, satellite and cable communication networks In order to get better information more effectively in the modern society, the authors needed to actively deal with multimedia contents provided by new media system, such as video-conferencing, distance education, Internet broadcasting, and things go on.This was the way to satisfy customers desire for better services in medical area. Consequently, it became possible to practice telemedicine, including distance medical examination and treatment, counseling, and education. The updated networksystems using ISDN, optical lines, microwaves and multi-channel satellite can be utilized for a video-conferencing system to support an effective telemedicine. In order to establish and execute national information policies effectively, it is requiredthat the laws, regulations, and the organizations related to broadcasting and communication matters need to be modified.The purpose of this paper is to suggest a model of a medical web- casting system with high cost-effectiveness. This has been supported by our experience in establishing an internet broadcasting system at a general hospital.