Influence of physical activity and different sensory conditions on static and dynamic balance of pregnant women

Abstract BACKGROUND Physical activity during pregnancy provides countless benefits to pregnant women and results in a smaller number of falls during pregnancy. Thus, this study aims to verify the influence of physical activity and test conditions on the static and dynamic balance of pregnant women. METHODOLOGY The study was an analytical, transversal, and comparative study with a sample of 99 pregnant women divided into two groups, with physical activity as the grouping factor. The evaluation included questions about the type and frequency of physical activity and investigation of postural balance (Balance Master System(r)). For statistical analysis, we used the following tests: Shapiro Wilk, Mann-Whitney, ANOVA One Way and Wilcoxon. RESULTS No relationship was found between physical activity and postural balance (F > 0.40, P > 0.07). However, the test conditions alter the sway velocity (P = 0.001) and directional control movement (P = 0.001). CONCLUSIONS Results suggest that: (1) physical activity does not influence variables related to postural balance in active and sedentary pregnant women; and (2) postural sway velocity and directional control of movement are related to the test conditions used during balance evaluation.

Ultrastructure of Zika virus particles in cell cultures

Zika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production, and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron microscopy (TEM). Blood was obtained from a Brazilian patient during the first days after presenting with signs of the disease, and ZIKV from the patient’s blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible.

Assessment of enamel-dentin caries lesions detection using bitewing PSP digital images

OBJECTIVES: The aim of this study was to evaluate the detection of enamel-dentin occlusal caries using photostimulable phosphor plates. MATERIAL AND METHODS: The ability to detect enamel-dentin occlusal caries in 607 premolars and molars from 47 patients between 10 and 18 years old, referred to the School of Dentistry of the Federal University of Bahia, Brazil, was evaluated based on clinical and radiographic examinations, using the criteria proposed in a previous study. A total of 156 bitewing digital images were obtained using Digora® (Soredex Medical Systems, Helsinki, Finland) phosphor plates. The plates were scanned and the images were captured and displayed on a computer screen. Image evaluation was done using Digora® for Windows 2.1 software, Soredex®. The radiologists were allowed to use enhancement tools to obtain better visibility during scoring of the teeth based on the radiographic criteria proposed in a previous study. Descriptive analysis and chi-squared proportion tests were done at 5 percent significance level. RESULTS: The results of clinical examination showed a higher prevalence of teeth with a straight dark line or demineralization of the occlusal fissure (score 1) and a lower prevalence of sealed teeth (score 5). In the bitewing digital images, 47 teeth presented visible radiolucency, circumscribed, in dentin under occlusal enamel (enamel-dentin caries lesions). CONCLUSIONS: Correlating the clinical and radiographic findings, it was found that in the majority of teeth diagnosed by radiographic images as having enamel-dentin caries, no caries could be detected by clinical examination.