Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window

Abstract Purpose: To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cell Methods: 16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphate-buffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal). After the treatments were performed, at 8 and 31 days, four animals in each group were subjected to left nephrectomy for histological studies. Results: Histologically, a higher amount of cell debris and tubules devoid of the epithelium and a higher degree of necrosis were observed in the groups treated with PBS, as opposed to a low degree of necrosis and higher tubular vascularization in the groups treated with ADSC, particularly in the group treated with intrarenal ADSC 48 hours after injury. Conclusion: The transplantation of ADSC positively contributed to the replacement of necrotic tissue by renal tubular cells, vascularization of the renal parenchyma, and restoration of the organ function.

Lesões meniscas em pequenos animais / Lesiones d menissco en pequeños animales / Menscal injuriess in small animals

Os meniscos têm a função de diminuir a força de impacto e aumentar a estabilidade articular e a congruência articular da articulação do joelho. São estruturas semilunares, de constituição fibrocartilaginosa e em grande parte avascularizadas apresentando uma difícil cicatrização mediante uma injúria. Em cães, a lesão de menisco geralmente é secundária à ruptura de um ou mais ligamentos, principalmente, pela ruptura do ligamento cruzado cranial, e o menisco medial, o mais comumente lesionado, devido a sua pouca mobilidade. Independentemente da sua origem, mecanismo ou extensão, as lesões meniscais são tratadas com o objetivo de restaurar a integridade anatômica e funcional do menisco, visando à restauração da biomecânica articular. O presente artigo objetivou realizar uma revisão de literatura a respeito das lesões meniscais em cães e as opções terapêuticas disponíveis na prática da medicina veterinária.

The meniscus play a role in reducing the impact force and increasing joint stability and congruity of the knee joint. They are semilunar strucutes, with fibrocartilaginous constitution, and are largely avascularized. Therefore, they present difficulty in healing in face of an injury. In dogs, meniscus injury is usually secondary only to the rupture of one or more ligaments, mainly by the rupture of cranial cruciate ligament. The medial meniscus is the most commonly injured one due to its low mobility. Regardless of the origin, mechanism or extension, meniscus injuries are treated with the objective of restoring the anatomical and functional integrity of the meniscus, aiming the restoration of joint biomechanics. This paper aims to review the literature regarding meniscal injuries in dogs and the treatment options available in the practice of veterinary medicine.

Los meniscos tienen la función de disminuir la fuerza del impacto y aumentar la estabilidad articular y la congruencia articular de la articulación de la rodilla. Son estructuras similares de constitución fibrocartilaginosa y en gran parte avascularizado presentando difícil cicatrización mediante a una lesión. En perros, la lesión de menisco generalmente es secundaria a la ruptura de uno o más ligamentos, principalmente, por la ruptura del ligamento cruzado craneal, y el menisco medial lo más comúnmente lesionado, debido a su poca movilidad. Independientemente de su origen, mecanismo o extensión, las lesiones de menisco son tratadas con el objetivo de restaurar la integridad anatómica y funcional del menisco, pretendiendo la restauración de la biomecánica articular. Este artículo objetivó realizar una revisión de literatura a respecto de las lesiones de menisco en perros y las opciones terapéuticas disponibles en la práctica de la medicina veterinaria.

Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice / Associação de células-tronco mesenquimais com plasma rico em plaquetas na reparação de defeitos críticos em calvária de camundongos

PURPOSE: To evaluate the effects of mesenchymal stem cells (MSC) from eight mice C57BL/6 gfp+ bone marrows expanded in cultures associated with platelets rich plasma (PRP) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6. METHODS: The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL associated with 50.0µL of plasma gel containing 1.0 x 10(9) autologous platelets within the defect. RESULTS: In the treated group was observed process of angiogenesis and bone repair better than control group. CONCLUSION: Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp+ mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.

OBJETIVO: Avaliar os efeitos da associação das células-tronco mesenquimais (MSC) oriundas da medula óssea de oito camundongos jovens C57BL/6 gfp+ e expandidas em culturas, com Plasma Rico em Plaquetas (PRP) provenientes de outros oito camundongos, na reparação de defeitos críticos confeccionados em calvária de 24 camundongos adultos C57BL/6. MÉTODOS: Os animais foram submetidos a um defeito craniano de 6,0mm de diâmetro e separados em dois grupos experimentais iguais. O grupo controle não recebeu tratamento e no grupo tratado foi administrado, no interior do defeito, pellet de MSC contendo 1,0 x 10(7) células/mL associado com 50,0µL de plasma em gel autólogo contendo 1,0 x 10(9) plaquetas. RESULTADOS: No grupo tratado verificou-se processo de angiogênese e reparação óssea superior ao grupo controle. CONCLUSÃO: A associação das células-tronco mesenquimais (MSC) derivadas da medula óssea de camundongos C57BL/6 gfp+ com gel de PRP aplicadas em defeitos ósseos críticos confeccionadas em calvária de camundongos C57BL/6 jovens, contribuiu positivamente para o processo de reparação óssea.