Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene

An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome B (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. The complete cytb gene sequences of 11 vertebrate species available in the National Center for Biotechnology Information database were digested with Aci I, Alu I, Hae III and Rsa I restriction enzymes in silico using Restriction Mapper software. The cytb gene fragment (358 bp) was amplified from tissue samples of vertebrate species and the dietary contents of sandflies and digested with restriction enzymes. Vertebrate species presented a restriction fragment profile that differed from that of other species, with the exception of Canis familiaris and Cerdocyon thous. The 358 bp fragment was identified in 76 sandflies. Of these, 10 were evaluated using the restriction enzymes and the food sources were predicted for four: Homo sapiens (1), Bos taurus (1) and Equus caballus (2). Thus, the PCR-RFLP technique could be a potential method for identifying the food sources of arthropods. However, some points must be clarified regarding the applicability of the method, such as the extent of DNA degradation through intestinal digestion, the potential for multiple sources of blood meals and the need for greater knowledge regarding intraspecific variations in mtDNA.

A gap analysis of ornithological research in the brazilian state of Roraima / Análise de lacunas da investigação ornitológica no estado de Roraima, Brasil

This study analyzes the ornithological data available for the Brazilian state of Roraima, focusing on sampling gaps. All ornithological data collected in Roraima up to the present were collated with the aim of answering the following questions: (a) which sites have been well sampled? (b) Are there are any geographic gaps? (c) What is the current extent of the scientific knowledge of avian diversity in Roraima? (d); Which regions and vegetation types have been well-sampled, and which should be prioritized for future surveys? Ornithological data were obtained from a total of 82 localities, including the Maracá Ecological Station (442 species), Viruá National Park (420 species), Colônia do Apiaú (320 species), Mucajaí (267 species), and Pacaráima (212 species), which were the five sites with the highest species counts. Over the past 20 years, only two localities in Roraima have been added to the list of sites with reliable samples (at least 100 bird species recorded (study skins collected, tape recordings and undocumented field observations) , the Viruá National Park and Fazenda Paraense. Five regions of the state were identified as sampling gaps, and should be prioritized in future ornithological surveys: the northwest, on the border with Venezuela and the Brazilian state of Amazonas, the lower Rio Branco between the town of Caracaraí and the mouth of the river, the terra firme forests of the southeast, the savannas of the northeast, on the border with Guyana, and the northern highlands, including montane forest and tepuis.

Neste artigo, o esforço de investigação ornitológica no estado de Roraima será investigado utilizando abordagens de análises de lacunas. Basicamente, apresentaremos uma síntese sobre todo o esforço ornitológico feito até o momento em Roraima, visando responder as seguintes questões: (a) quais os locais bem amostrados para aves? (b) quais são as lacunas geográficas de investigação? (c) em que estágio de descobertas está o inventário das espécies de aves em Roraima? (d) quais são os macro-hábitats prioritários para investigação? (e) quais os tipos de vegetação bem investigados e quais os que podem ser classificados como prioritários para investigação? Como resultados, detectamos Oitenta e duas localidades com algum tipo de informação ornitológica. Dentre essas, a Estação Ecológica de Maracá (442 sp), seguido pelo Parque Nacional do Viruá (420 sp), Colônia do Apiaú (320 sp), Mucajaí (267 sp), e Pacaráima (212 sp), são as cinco localidades com maior grau de conhecimento ornitológico dentro do estado. Nos últimos 20 anos apenas duas localidades ornitológicas em Roraima podem ser acrescidas à lista de sítios bem estudados de acordo com o critério de pelo menos 100 espécies registradas (peles, gravações de voz e observações visuais): Parque Nacional do Viruá e a Fazenda Paraense. Cinco áreas são aqui apontadas como lacunas de amostragem da avifauna de Roraima e devem receber prioridade para novos inventários e estudos sobre a avifauna dessa região, são elas: Noroeste do estado, nas áreas junto à fronteira com a Venezuela e divisa com o Estado do Amazonas; Baixo Rio Branco, desde a cidade de Caracaraí à sua foz; Florestas de terra firme no sudeste do Estado; As savanas do nordeste junto à fronteira com a Guiana e as regiões de floresta montana e os tepuis.