RESUMO
Electrophoretic studies were carried out on isozymes of 3 populations of Anopheles minimus collected from Guangxi and Yunnan Provinces of the People's Republic of China in 1993. Eight proteins were analyzed by 5% polyacrylamide gel electrophoresis. The most variable population, 'Yunnan-Field' (Y-F), was highly polymorphic at 14 of 20 loci (P = 0.700) with an average heterozygosity H of 0.340. P values of 0.500 and 0.700, and H values of 0.220 and 0.210 were obtained for each from 'Guangxi-Lab' (GX-L) and 'Yunnan-Lab' (Y-L), respectively, Nei's genetic distances (D) between Y-L and GX-L, Y-F and GX-L, and Y-F and Y-L were 0.1131, 0.1946 and 0.1069, respectively. These results suggest that GX-L is distant from the 2 other populations, Y-L and Y-F, and that this genetic differentiation between the 2 populations of Yunnan and Guangxi Provinces corresponds to the forms A and B, which were morphologically classified by Xu et al (unpublished).
Assuntos
Animais , Anopheles/genética , China , Eletroforese em Gel de Poliacrilamida , Feminino , Frequência do Gene , Variação Genética , Genética Populacional , Isoenzimas/genéticaRESUMO
Electrophoretic studies were carried out on isozymes of 3 populations of Anopheles minimus collected from Guangxi and Yunnan Provinces of the People's Republic of China in 1993. Eight proteins were analyzed by 5% polyacrylamide gel electrophoresis. The most variable population, Y-F, was highly polymorphic at 14 of 20 loci (P=0.700) with an average heterozygosity H of 0.340. P values of 0.500 and 0.700, and H values of 0.220 and 0.210 were obtained for each from 'Guangxi-Lab' (GX-L) and 'Yunnan-Lab' (Y-L), respectively. Nei's genetic distances (D) between Y-L and GX-L, Y-F and GX-L, and Y-F and Y-L were 0.1131, 0.1946 and 0.1069, respectively. These results suggest that GX-L is distant from the 2 other populations, Y-L and Y-F, and that this genetic differentiation between the 2 populations of Yunnan and Guangxi Provinces corresponds to the forms A and B, which were morphologically classified by Xu et al (unpublished).
Assuntos
Alelos , Animais , Anopheles/genética , China , Eletroforese em Gel de Poliacrilamida , Variação Genética , Proteínas de Insetos/genéticaRESUMO
The genetic difference between Angiostrongylus malaysiensis and A. cantonensis was assayed by electrophoretic analysis of isozymes. Six enzymes were analyzed using 5% polyacrylamide gel electrophoresis. Seven of 10 loci, namely GPI-1, GPI-2, HK-1, HK-2, MDH-1, MDH-2 and PGM-2, were shown to be polymorphic, but the remaining 3 loci, LDH, ME and PGM-1, were not. Both A. malaysiensis and A. cantonensis were polymorphic at 6 of the loci (p = 0.600) with heterozygosity H of 0.286 and 0.151, respectively. The Nei's genetic distance (D) between A. malaysiensis and A. cantonensis was 0.27470. This value indicates the level of interspecific variation within a genus. Through isozyme analysis, the present study demonstrated that A. malaysiensis of Japan is a valid species, separate from A. cantonensis.