RESUMO
Total phenolic contents [TPC] of five different extracts [petroleum ether, dichloromethane. ethyl acetate, ethanol and ethanol-water] ofArtemisia biennis Willd were measured in this work. The antioxidant activity was investigated by three different methods: p-carotene bleaching [BCB] test, 2.2-diphenyl-l-picrylhydrazyl [DPPH] radical scavenging method and ferrous ion chelating [FIC] assay. Among all the extracts analyzed, the hydroethanolic extract exhibited a significantly higher phenolic content and antioxidant activity than other samples. Vacuum liquid chromatography of this extract yielded seven fractions [A to G] which were subjected to all aforementioned experiments. The highest total phenolic content and free radical scavenging activites were present in the same sample [Fraction D] but the only statistically significant correlation between TPC and EC. values was observed for BCB