RESUMO
Se analizan las dificultades de algunos profesionales sin experiencia en la compaginación y redacción de un trabajo médico procurando dar alguna orientación útil a tal fin. Se considera especialmente el trabajo de investigación clínica o científica al igual que la presentación casuística. Se destaca la importancia del protocolo previo y la correcta selección bibliográfica para su compaginación. En "redacción" se comentan aspectos útiles en título - autores - introducción - metodología - resultados - discusión - conclusiones - resumen y bibliografía. Se tratan normas generales que pueden ser de utilidad al autor novel, quedando, dada la amplitud del tema, aspectos sin considerar.
Assuntos
Redação/normas , Publicação Periódica/ética , Publicação Periódica/normas , Publicação Periódica/tendências , Pesquisa Biomédica , Revisão por Pares , Guias como AssuntoRESUMO
INTRODUCTION: The action of non-steroidal anti-inflammatory drugs (NSAIDs) on the Helicobacter Pylori (Hp) infected mucosa is a matter of debate. Some authors consider them to cause additive iatrogeny whilst others attribute a purportedly protective action to them. The development of on experimental animal model could help clarify this phenomenon. OBJECTIVES: 1--To develop an animal model of Hp gastric infection. 2--To evaluate the aggressiveness of NSAIDs in this model. MATERIALS AND METHODS: Male 6 month old BALC/C mice weighing 38 g were studied. Pylori Hp infection was ruled out. On three occasions, in the same week, 18 mice were inoculated intra-gastrically with 0.6 ml of Hp culture broth (brain-heart infusion) containing 1 x 10 8-1 x 10 9 CFU/ml. Another group of mice were inoculated with sterile saline. After two months the mice were killed and their stomachs studied. They were divided into groups: a) 6 Hp negative control mice. b) 8 Hp negative mice with prior intra-peritoneal injection of 25 mg/Kg indomethacin (24 hs.) c) 8 mice inoculated with Hp with indomethacin. d) 8 mice inoculated with Hp, without indomethacin. The stomachs were opened along the greater curvature and photographed macroscopically in order to map the necrotic area. The antrums were biopsied to test for urease and separate antrum and body specimens were send for staining with Warthin-Starry H & B and histopathology. RESULTS: All the mice inoculated with Hp acquired the infection. The necrotic area was larger in Group B: 55.5 +/- 7.87 mm than in Group C: 15 +/- 1.82 mm P < 0.00019. HISTOLOGY: Group A: normal mucosa. Group B: extensive coagulation necrosis and focal erosions. Group C: ulcers with inflammatory infiltrate and smaller necrotic area, presence of Hp on the surface epithelium. Group D: no ulcers, Hp present. CONCLUSION: An animal model of Hp infection was successfully developed Hp infection could play a potentially protective role against indomethacin aggression in the mouse.
Assuntos
Animais , Masculino , Anti-Inflamatórios não Esteroides , Modelos Animais de Doenças , Mucosa Gástrica , Infecções por Helicobacter , Helicobacter pylori , Indometacina , Mucosa Gástrica , Camundongos , Camundongos Endogâmicos BALB CRESUMO
A 70 pacientes divididos en tres grupos: A) Control (14); B) Ulceras Gástricas (31), C) Gastritis Crónica (25) se les efectuó una determinación de R.D.G. con Cámara Gamma y Tc99 HIDA. Un segundo estudio se realizó a las 48-72 horas a 30 pacientes a los efectos de evaluar la reproductibilidad del método, la que alcanzó un 80%. En los pacientes con UG y CG se encontró la existencia de RDG en una proporción significativamente mayor que entre los controles sanos (P 0.01). A su vez la magnitud de dichos reflujos fue significativamente superior en las GC y en las UG tipo III. De acuerdo con estos resultados, se atribuye al R.D.G. un papel significativo en la patogenia de un porcentaje importante de pacientes con UG y GC