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Mem. Inst. Oswaldo Cruz ; 107(7): 899-902, Nov. 2012. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-656046

RESUMO

This study aimed to evaluate the occurrence of schistosomiasis in areas with low endemicity using polymerase chain reaction (PCR) as a diagnostic method. We analysed faecal samples from 219 individuals residing in Piau and Coronel Pacheco, state of Minas Gerais, Brazil, using a single faecal sample from each individual and two slides of the Kato-Katz technique as a gold standard. Fifteen out of the 219 samples were positive with both methods of diagnosis. One sample was diagnosed as positive by the Kato-Katz technique only and 61 were diagnosed only by PCR. The positivity rates were 7.3% with the Kato-Katz method and 34.7% with PCR. When both techniques were assumed to have 100% specificity and positive individuals were identified by both methods, the sensitivity of the Kato-Katz method was 20.8% and the PCR sensitivity was 98.7%. The Kappa index between the two techniques was 0.234, suggesting weak agreement. The assessment of a single faecal sample by PCR detected more cases of infection than the analysis of one sample with two slides using the Kato-Katz technique, suggesting that PCR can be a useful diagnostic tool, particularly in areas with low endemicity.


Assuntos
Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Pessoa de Meia-Idade , Adulto Jovem , Reação em Cadeia da Polimerase , Schistosoma mansoni/genética , Esquistossomose mansoni/diagnóstico , Brasil , Fezes/parasitologia , Valor Preditivo dos Testes , Sensibilidade e Especificidade , Schistosoma mansoni/isolamento & purificação
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