RESUMO
Objective: In order to find the suitable concentration and combination of plant growth regulators, the effects of plant growth regulators (NAA, 2, 4-D, 6-BA, KT, and PP333) on in vitro induction formation for the plantlet microtuber of Dioscorea bulbifera was studied. Methods: Through plant tissue culture technique, single factor test, and orthogonal test, taking the stems with a bud of D. bulbifera plantlets as explants, the effects of plant growth regulators on the in vitro induction formation for the microtubers of D. bulbifera were investigated. Results: Auxin using alone was conducive to the induction formation for the microtuber of D. bulbifera. The suitable concentration of both NAA and 2, 4-D inducing the microtuber formation was 0.5 mg/L, but the inducing effects of NAA and 2, 4-D had no significant difference. Cytokinin using alone was not conducive to the induction formation for the microtuber of D. bulbifera. The suitable concentration of both KT and 6-BA inducing microtuber formation was 2 mg/L, but the inducing effect of KT is better than that of 6-BA. The combination of auxin, cytokinin, and PP333 could significantly promote the in vitro induction formation for the microtuber of D. bulbifera, the better combination was MS+NAA 0.5 mg/L+6-BA 2.0 mg/L+PP333 0.5 mg/L. Conclusion: Based on these experimental results, the paper selects the suitable concentration of plant growth regulators conducive to the in vitro induction formation for the microtuber of D. bulbifera, which has laid the technical foundation for their in vitro induction formation of microtuber and factory production.
RESUMO
Objective: The tissue culture of Anemarrhena asphodeloides was preliminarily studied to establish A. asphodeloides regeneration system. Methods: The establishment of A. asphodeloieds sterile system, tiller bud proliferation, tiller callus induction and its re-differentiation as well as transplanting of regenerated plantlets were studied by plant tissue culture and single factor test method. Results: The best disinfection way of A. asphodeloides seeds was firstly dealt with 75% ethanol for 30 s and then dealt with 0. 1% HgCl2 for 15 min; The best medium of bud proliferation for A. asphodeloides tillers was MS+KT 1 mg/L+NAA 0.5 mg/L; The best medium of A. asphodeloides tiller callus induction was MS+KT 2 mg/L+NAA 0.5 mg/L; The best medium of A. asphodeloides tillers callus redifferentiation was MS+ KT 2 mg/L+NAA 0.1 mg/L; The best rooting medium of A. asphodeloides callus regeneration buds was 1/2 MS+NAA 0.5 mg/L; The best transplanting substrate of A. asphodeloides plantlets was humus soil. Conclusion: The regeneration system of A. asphodeloides is established, which provides a technological basis for factory production of A. asphodeloides plantlets.