Research advances on directional induction and differentiation in vitro from human pluripotent stem cells into erythrocytes / 中国实验血液学杂志

Red blood cell transfusion is an effective method to treat acute hemorrhage and severe anemia. However, blood source from donors is very limited, and transfusion-transmitted diseases occurred frequently, thus threatening human health. Therefore, the safe, abundant and functional blood source is needed. Generation of blood cells from human pluripotent stem cells(hPSC) will offer alternative approach. Lots of studies have been focused on erythroid cell differentiation in vitro, including how to enhance efficiency and improve their function. In this review, the research advances on differentiation methods and the regulatory mechanism are summarized. In addition, the progress in PSC differentiation into erythrocytes and the problems to be solved are discussed briefly.

Screening and identification of high-yield poly(β-malic acid) bacterial strain / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To isolate and identify the high-yield poly-malic acid (PMLA) bacterial strains from the nature.</p><p><b>METHODS</b>Samples were collected and cultured. The high-yield PMLA bacterial strains were screened through morphological observation, qualitative PMLA tests by HPLC and ITS sequence analysis on the isolated bacterial strains.</p><p><b>RESULTS</b>A high-yield PMLA strain II 04 was isolated, the yield of PMLA of the strain reached to 26.23g/L in the rotary shaker at 25 degree for 7d. From morphological observation and ITS sequences analysis, the strain belonged to Aureobasidium pullulans, and named as Aureobasidium pullulans ZUCC-41.</p><p><b>CONCLUSION</b>A high-yield bacterial strain has been isolated from the nature and identified to be Aureobasidium pullulans.</p>