Protective Effect of selenium-based medicines on toxicity of three common organophosphorus compounds in human erythrocytes in vitro

Objective: Organophosphorus [OP] compounds are used to control pests, however they can reach the food chain and enter the human body causing serious health problems by means of acetylcholinesterase [AChE] inhibition and oxidative stress [OS]. Among the OPs, chlorpyrifos [CHP], malathion [MAL], and diazinon [DIA] are commonly used for commercial extermination purposes, in addition to veterinary practices, domestic, agriculture and public health applications. Two new recently registered medicines that contain selenium and other antioxidants, IMOD and angipars [ANG], have shown beneficial effects for OS related disorders. This study examines the effect of selenium-based medicines on toxicity of three common OP compounds in erythrocytes

Materials and Methods: In the present experimental study, we determined the efficacy of IMOD and ANG on OS induced by three mentioned OP pesticides in human erythrocytes in vitro. After dose-response studies, AChE, lipid peroxidation [LPO], total antioxidant power [TAP] and total thiol molecules [TTM] were measured in erythrocytes after exposure to OPs alone and in combined treatment with IMOD or ANG

Results: AChE activity, TAP and TTM reduced in erythrocytes exposed to CHP, MAL and DIA while they were restored in the presence of ANG and IMOD. ANG and IMOD reduced the OPs-induced elevation of LPO

Conclusion: The present study shows the positive effects of IMOD and ANG in reduction of OS and restoration of AChE inhibition induced by CHP, MAL and DIA in erythrocytes in vitro

Synthesis and analgesic activity of novel hydrazide and hydrazine derivatives

The uses of non-steroidal anti-inflammatory drugs [NSAIDs] are limited by a variety of side effects. So research on preparing new analgesic agents is important. According to some reports about the analgesic activity of hydrazide and hydrazine derivatives a new series of these compounds were synthesized in order to obtain new analgesic compounds. The final compounds l0a-l0e and 15a-15d were prepared by condensation of corresponding hydrazides 7, 8 and 11-14 with different aldehydes 9a-9e. The structures of all synthesized compounds were confirmed by means of FT-IR, H-NMR and Mass spectra. All compounds were evaluated for their analgesic activities by abdominal constriction test [writhing test]. Most of the synthesized compounds induced significant reduction in the writhing response when compared to control and compound 15 was more potent than mefenamic acid in the writhing test