RESUMO
Background and Aim: the aim of this study was to investigate the effects of mobile phone electro-magnetic field [EMF] on testosterone level and testicular tissue in rats
Material and Methods: a total of 30 male mice were randomly divided into two equal groups [ n=15]. Experimental group was exposed to EMF, by using mobile phone continuously for 90 days. Control group was kept under the same condition as the experimental group without EMF exposure. Throughout the study cell phones were placed on the wall of the cages. The cell phones were in standby mode for whole day and in talking mode for 150 min per day. At the end of the exposure period, total testosterone serum level was measured and histological examination of testicular sections were performed. Using GraphPad Prism' software, data were analyzed by t-test. P<0.0001 was considered significant
Result: mean plasma testosterone level in the experimental group was decreased compared to that in the control group [P<0.0001]. Histological examination of testicular tissue of the mice exposed to electromagnetic field revealed seminiferous tubules cellular necrosis, infiltration of inflammatory cells into the paranchymal tissue, and decreased leydig cell count at interstitial space. In some necrotized tubules, multinucleated cells [syncytium formation] were observed
Conclusion: the results of this study indicated that exposure to mobile phone electromagnetic waves could result in histopathological changes in the male mice testis. Further studies can cast light on the molecular mechanism underlying cellular and tissue damage induced by exposure to electromagnetic radiation
RESUMO
Background and Objective: The electromagnetic field producer sets in daily life causes concerns about the adverse effects of such waves on human health. This study was done to evaluate the effect of cell phones microwaves on histologic structures of some visceral organs in rat
Methods: In this experimental study, 80 immature Wistar male rats with weight of 100-140 gram and 5 to 6 weeks age were randomly allocated into 3 experimental groups and one control group. The experimental rats were exposed to cell phones microwaves 5 hours a day for 1, 2 and 3 months. The control group received no radiation. After the experimental period rats were sacrificed and the appropriate tissues of the lung, heart, liver, brain and pancreas were prepared. Sections in 5 micro m thicknesses were stained by hematoxylin and eosin and studied microscopically
Results: Histological changes including edema, inflammatory cell infiltration and cell degeneration in the lung and mild degeneration and coagulation necrosis of the myocardial cells in the heart were observed. Histopthological examination of the liver revealed dilation of central veins and sinusoids, vacuolization of hepatocytes and mononuclear inflammatory cell infiltration. Limited changes were observed in pancreas and brain. Histological changes were increased in the groups that were exposed longer period of time to radiation
Conclusion: This study indicated that the harmful effect of cell phones radiation on rat tissues depending on the duration of exposure