RESUMO
Cavernous hemangiomatous polyp of uterus is a rare vascular malformation of the uterus. Common sites of malformation in uterine wall include serosa, myometrium, and endometrium and most common presentation is diffuse involvement of uterus. Patients usually present with obstetric and gynecological complications ranging from menorrhagia, intermenstrual bleeding, infertility, and pregnancy‑associated complications which includes maternal and fetal demise due to excessive bleeding. Uterine hemangiomas occur at all age groups without preponderance in any decades and are classified into congenital and acquired. Congenital hemangiomas may be associated with hereditary diseases whereas acquired hemangiomas are thought to be associated with both physical changes and hormone alterations.
RESUMO
The antifungal potential of medicinal plant Tylophora indica Merr. was evaluated by agar well diffusion method and it was compared with the activity of its in vitro raised plant and callus . The extracts of in vitro raised plant and callus showed better antifungal activity against the tested fungal species as compared to parent plant. Minimum inhibitory concentrations (MIC) of the extracts were determined by broth microdilution method. The MIC of the alcoholic leaf extracts of parent plant against tested fungi ranged from 12.0 to 98.0 μg/ml, whereas, the MIC of extract of in vitro raised plant and callus ranged from 1.53 to 49.0 μg/ml and 3.05 to 24.0 μg/ml respectively. The present study leads to conclusion that extracts of Tylophora indica contain good antifungal activity which could be used in the treatment of various fungal infections showing resistance to treatment by currently used antifungal agents. As the in vitro raised plant and callus gave good results, in vitro cultivation of the explants may be used to obtain novel antifungal compounds. This is the first report on antifungal activity of Tylophora indica through in vitro raised plant and its callus.
RESUMO
Staphylococcus aureus, the main cause of nosocomial infection worldwide result in significant increases in mortality, morbidity, and cost related to prolong treatments. Silver compound has been in use since time immemorial for the treatment of burns, wounds and several other bacterial infections. In the present work, we explore the antibacterial activity of silver nanoparticles (Ag-NPs) dispersion (5-10 nm) against reference strain and clinical isolates of Methicillin-sensitive S. aureus (MSSA), and Methicillin-Resistant S. aureus (MRSA).The typical minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against standard reference strain as well as, MSSA and MRSA were observed in the range of 12-48 μg/ml and 12-96 μg/ml, respectively. The MBC/MIC ratios against all strains were found in the range of ≤1 to ≤4, which shows that Ag-NPs inhibit bacterial growth in a bactericidal rather than a bacteriostatic manner. Our finding suggests that Ag-NPs are effective broad-spectrum antibacterial agents regardless of their drug-resistance mechanisms.
RESUMO
Apoptotic effects of curcumin (diferuloyl methane) on squamous cell carcinoma of the cervix. The present study was conducted in departments of Pathology and Biochemistry, JNMC, AMU, over a period of one and half yrs. Caspase-3 and TNF-alpha assay was performed on monocytes isolated from cervical carcinoma patients and cultured with curcumin; cytosmears and sections from cervical carcinoma tissue cultured with curcumin were prepared for the morphological evidence of apoptosis. Curcumin in the doses of 500 microg/ml increased the caspase-3 levels and decreases the level of TNF-alpha in human cells. Cytosmears and sections from cervical carcinoma tissue cultured with curcumin showed better differentiation and increased number of apoptotic cells as compared to non curcumin controls. Curcumin in a dose of 500 microg/ ml promoted apoptotic preparedness of human cells and induced apoptotic change in cervical carcinoma cells.