RESUMO
The objective of present study was to develop and evaluate polyethylene glycol [PEG] based diclofenac sodium suppositories. This study used water soluble PEG bases [1000, 4000 and 6000] in different combinations to formulate suppositories, which were further subjected for their physicochemical properties evaluation such as weight variation, average melting point, content uniformity and disintegration. Dissolution test was used to perform the in vitro release rate studies of the suppositories. The suppository [P3] containing PEG-6000 [50%] and PEG-4000 [50%] exhibited rapid in vitro release rate of diclofenac sodium. Moreover, homogeneous distribution of diclofenac sodium is found in all six formulations. The in vitro release patterns of diclofenac sodium from the marketed Voltral suppository [100mg] and formulated suppositories were also compared and found in standard limits
RESUMO
Candesartan [CAN], an ARB-blocker, antihypertensive, was analyzed in human plasma by a simple, accurate and precise RP-HPLC [reverse phase-High performance liquid chromatography assay method which was then validated for its accuracy, specificity and precision. The mobile phase has a constitution of acetone, diethylamine and distilled water, while Phosphoric acid was used to adjust the pH to 2.5 +/- 0.1. This mobile phase was run at 1.1ml/min and the fluorescence wavelength was set to 392 nm. A C-18 HPLC, column particle size [5 [micro]m] Mediterranean Sea ® L x 1.D. 25cm x 4.6 mm [Supelcosil] , with auto sampler injection volume of 30[micro]l ,an internal standard Valsartan was utilized for chromatographic detection. Candesartan took a retention time of 6 +/- 0.5 minutes. This method was validated by the parameters of selectivity, accuracy, precision, repeatability, reproducibility, recovery, linearity and stability. Candesartan's calibration curves were found to be linear in the range of 200ng/ml to 3.125ng/ml and the coefficient of determination [r2] was found to be 0.99. Analytical recovery obtained was above 88%. Hence, this method has been found to be useful for determining Candesartan in plasma
RESUMO
The. effect of food on the physiological availability of ampicillin was investigated in a single-dose study in healthy human subjects. The bioavailability of ampicillin was determined using the plasma levels at different sampling times. Plasma ampicillin levels were analyzed assuming a one-compartment linear model with first order absorption and elimination. The results indicate a reduction in ampicillin absorption as evidenced by peak plasma levels [3.20 ug/ml fasting, 2.98 ug/ml nonfasting] and area under the plasma level versus time curve [11.51 ug/ml hr fasting, 8.24 ug/ml. hr, nonfasting]. These results confirm that the presence of food causes a substantial decrease and delay in the absorption of ampicillin
Assuntos
Ingestão de AlimentosRESUMO
The influence of fluid volume on the relative bioavailability and pharmacokinetics of ampicillin from a solid dosage form has been studied in healthy male volunteers. Plasma ampicillin levels were analyzed assuming a one-compartment linear model with first order absorption and absorption delay. The results showed little or no effect of dehydration versus normal state on ampicillin absorption as evidenced by peak plasma levels [3.10 micro g/ml dehydration, 3.11 micro g/ml normal state] and area under the plasma level versus time curve [12.49 micro g/ml-hr dehydration, 11.13 /micro g/ml-hr normal state]. These results confirm the reliable absorption of orally administered ampicillin in water deprivated as well as in normal state