Development and validation of selective UV spectrophotometric analytical method for budesonide pure sample.

Budesonide is a very potent corticosteroid, used for bronchial asthma and inflammatory bowel disease. Objective of the present investigation is to develop the simple and selective UV spectrophotometric method for quantification of budesonide in bulk sample. Absorption maximum of budesonide was found to be 246.0 nm and obeyed the beers law in the concentration range of 1.4 to 25 μ g/ml. Calibration curve shows a linear relationship between the absorbance and concentration in the range of 2 to 10 μ g/ml and the limit of detection is 0.01 μ g/ml. The limit of quantification was found to be 1.4 μg/ml. The method was validated for repeatability, accuracy and precision. The percent amount of recovery was 99 - 100% with minimum standard deviation less than 1%. Obtained results showed there is minimum intra day and inter day variation. The excipients present in the preparation did not interfered during the analysis. Developed analytical UV spectrophotometric method is simple, rapid and reproducible and further it can be used for estimation of drug in bulk and colon matrix tablet dosage form.

Nootropic activity of tuber extract of Pueraria tuberosa (Roxb).

Nootropic effect of alcoholic (ALE; 50, 75, 100 mg/kg) and aqueous (AQE; 100, 200, 400 mg/kg) extracts of P. tuberosa was evaluated by using Elevated Plus Maze (EPM), scopolamine-induced amnesia (SIA), diazepam-induced amnesia (DIA), clonidine-induced (NA-mediated) hypothermia (CIH), lithium-induced (5-HT mediated) head twitches (LIH) and haloperidol-induced (DA- mediated) catalepsy (HIC) models. Piracetam was used as the standard drug. A significant increase in inflexion ratio (IR) was recorded in EPM, SIA and DIA models. A significant reversal effect was observed on rectal temperature in CIH model, reduction of head twitches in LIH models. However no significant reduction in catalepsy scores in HIC models were observed with test extracts and standard piracetam. The results indicate that nootropic activity observed with ALE and AQE of tuber extracts of P. tuberosa could be through improved learning and memory either by augmenting the noradrenaline (NA) transmission or by interfering with 5-hydroxytryptamine (5-HT) release. Further, the extracts neither facilitated nor blocked release of the dopamine (DA). Thus ALE and AQE elicited significant nootropic effect in mice and rats by interacting with cholinergic, GABAnergic, adrenergic and serotonergic systems. Phytoconstituents like flavonoids have been reported for their nootropic effect and these are present in both ALE and AQE extracts of tubers of P. tuberosa (Roxb) and these active principles may be responsible for nootropic activity.