Effect of HLA-G expressed in platelets on Tax protein of human T lymphocyte leukemia type 1 virus / 中国输血杂志

【Objective】 To investigate the effect of HLA-G expressed in platelets on Tax protein of human T cell leukemia type 1 virus (HTLV-1). 【Methods】 Platelets were isolated from anticoagulant whole blood, and HLA-G molecule on platelet membrane was detected by flow cytometry. The content of secretory HLA-G before and after platelet lysis was detected by ELISA, HTLV-1 human lymphoma cells MT2 were cultured with platelet lysate (PL). The effect of HLA-G in platelets on the expression of HTLV-1 protein Tax was evaluated by Western blot (WB). 【Results】 Membrane type mHLA-G was highly expressed on the surface of platelet membrane. The expression of secretory sHLA-G (ng/mL) increased after platelet lysis (15.73±1.01) vs (6.65±0.47), the expression of sHLA-G increased with the increase of platelet concentration in a dose-dependent manner. Compared with fetal bovine serum, PL significantly promoted the high expression of HLA-G protein and HTLV-1 virus tax protein in MT2 cells, and the addition of anti-HLA-G antibody to PL could effectively inhibit the expression of Tax and HLA-G protein. 【Conclusion】 High expression of immune tolerance molecule HLA-G on platelets can induce high expression of HTLV-1 protein Tax in human lymphoma cell MT2, which contributes to viral infection.

Effects of HTLV-1 protein Tax on HLA-G expression / 中华微生物学和免疫学杂志

Objective:To study the expression of human leukocyte antigen G (HLA-G) during human T lymphocytic leukemia virus type 1 (HTLV-1) infection, and to investigate the function and mechanism of HLA-G in HTLV-1 immune escape.Methods:HeLa and THP-1 cells were infected by MT2, a stable HTLV-1 infected cell line. Expression of HLA-G isomers at mRNA and protein levels was detected by qRT-PCR and Western blot, respectively. Flow cytometry was used to detect the expression of HLA-G1. Moreover, transfection and siRNA gene were respectively used to up-regulate and silence HLA-G expression in HeLa cells to observe the changes in the expression of HTLV-1-featured protein Tax on protein level after HTLV-1 infection.Results:HTLV-1 infection could induce differential expression of HLA-G isomers, mainly HLA-G1 and HLA-G5, in HeLa and THP-1 cells. HLA-G expression at both mRNA and protein levels was significantly up-regulated 24 h after HTLV-1 infection. Moreover, the expression of HTLV-1 protein Tax was significantly enhanced in HTLV-1-infected HeLa cells overexpressing HLA-G. An opposite result was obtained when the HLA-G gene in HeLa cells was silenced by siRNA.Conclusions:The immune-tolerant molecule HLA-G was significantly increased in HTLV-1-infected cells, thereby promoting the expression of HTLV-1 viral protein, which led to persistent viral infection.

Clinical outcome of two operation methods in the treatment of degenerative spondylolisthesis / 中国矫形外科杂志

0.05);the better result was obtained including low back pain,Taillard index and relative disc height at the end of follow-up in POLA.[Conclusion]Posterior oblique lumbar arthrodeses is better ideal method in the treatment of degenerative spondylolisthesis.