Door-to-needle time for intravenous thrombolysis in acute ischemic stroke / 国际脑血管病杂志

Intravenous thrombolysis is an effective treatment for acute ischemic stroke, but its benefits are time-dependent. The time from onset to intravenous thrombolysis is divided into onset-to-door time (ODT) and door-to-needle time (DNT). The former reflects pre-hospital delay, while the latter reflects in-hospital delay and can be controlled by stroke improvement plan. This article reviews the influence of DNT on clinical outcomes, the influencing factors of DNT and the stroke improvement plan to shorten DNT.

Exploration on "three eye-needling" technique of acupuncture / 中国针灸

The "three eye-needling" technique is one of the important component of's three needling therapy, mainly used for the treatment of eye disorders such as optic atrophy, macular pigment degeneration, myopia, hyperopia, strabismus, amblyopia, diplopia, glaucoma, cataract, etc. In the paper,'s "three eye-needling" technique is explored, including the keys of manipulation, operation procedure and basic skills. This technique is particularly for "mind regulation", focusing on tranquilizing, observing and concentrating the mind. The precise selection of point is the basic requirement, the technique for fixing, pressing and pricking are the most important link. The needle insertion with one hand is adopted with gentle rotation manipulation. Mind regulation, point selection and specific operation are coordinated with each other to bring the function of "three eye-needling" technique into play and achieve better therapeutic effects.

CONSTRUCTION AND CHARACTERIZATION OF GOLDEN HAMSTER NEURAL TUBE cDNA LIBRARY / 解剖学报

Objective Constructing golden hamster neural tube cDNA library in order to detect new genes associated with development of neural tube and neural tube defects. Methods Total RNAs were isolated from neural tube using TRIZOL Reagent; Doublestranded cDNAs were synthesized from total RNAs using LD-PCR method; The dsc DNAs were digested by proteinase K and Sfi Ⅰ, less than 500?bp fragments were separated by CHROMA SPIN-400, and then the cDNAs were ligated to ? TriplEX-2 vector.After packaging,the neural tube cDNA library was constructed. Results The titer of neural tube cDNA library was 1.5?10 6?pfu/ml.The recombination rat was 99%.The average insert was larger than 1?kb.Conclusion Our successfully constructed cDNA library is a full-length library with high efficiency,and can be used to detect the genes related to development of neural tube and neural tube defects.

IMMUNOLOCALIZATION OF TGF?1 AND ITS RECEPTOR IN MOUSE ENDOMETRIUM DURING THE PROCESS OF IMPLANTATION / 解剖学报

Objective\ To further clarify the biological effects of TGF?1 on blastocyst implantation. Methods We used female adult Kunming mice to mate with male for observing the distribution of TGF?1 and its receptor at different stages of implantation with immunohistochemical method. Results\ Compared with nonpregnancy,TGF?1 and its receptor immunostaining markedly increased in epithelial and glandual cells on day 4 of pregnancy.The moderate extracellular staining of TGF?1 was present around the fibroblasts.On day 5\|6 of pregnancy,strong immunostaining in intensity of TGF?1 and its receptor was noted in primary decidual zone.With blastocyst invasion,TGF?1 and its receptor immunostaining diffused in decidual cells. Conclusion\ TGF?1 might play an important role via its receptor during blastocyst implantation.\;