RESUMO
Objective To investigate the feasbility of the orient differentiation from embryo hepatic Sca-1+ cell to hepatic cell or hepatic cell precursor under the induction of hepatocyte growth factor(HGF) and acor fibroblast growth factor(aFGF) in vitro. Methods Sea-1+ cells were isolated by immunomagnetic beads and their morphous were observed by contrast phase microscope. The expression of mRNA of albumin(ALB) and meta-thyroprotein were detected by RT-PCR; ALB, AFP, CK8/18 protein were detected by immunohistochemical method, and cell staining for glycogen and urea synthesis function were tested. Results The activity, purity, recovery rate of Sca-1+ cells were (94. 24±1.04) %, (85.57±1.66) %, (62. 31±1.85 ) % respectively. After the induction of HGF and aFGF, Sca1+ cells became anomalism and transformed to heptic cell in morphous,disappeared in expression of AFP protein and upregulated in expression of ALB and CK8/18 protein,upregulated in expression of ALB and transthyroprotein mRNA,cell staining of glycogen and urea synthesis function were strengthened,the cell differentiated to mature hepatic cell. Conclusion Embryo hepatic Sca-1+ cell could differentiate to hepatic cell or hepatic cell precursor under the induction of HGF and aFGF in vitro.