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Objective To study the action and mechanism of Sancai Lianmei Particle on cell apoptosis of liver cells in Type 2 Diabetes Mellitus(T2DM)combined with Non-alcoholic Fatty Liver Disease(NAFLD).Methods High fat and sugar + STZ induced diabetic with fatty liver rats used as models,intervention with Sancai Lianmei Particle,intraperitoneal glucose tolerance test(IPGTT)to assess insulin resistance,ELISA method to detect the mice serum biochemistry,insulin levels;ELISA method to detect inflammatory factors in liver homogenate;SOD and MDA levels were monitored to assess the degree of oxidative stress;ASK1/JNK/NF-κB mRNA expression in liver tissue was monitored by Real-time PCR;apoptotic-related proteins were detected by Western blot,apoptosis of hepatocytes was assayed by TUNEL;HE staining was conducted to observe the liver tissue.Results Sancai Lianmei Particle can obviously reduce the body weight of T2DM with NAFLD model rats;reduce the levels of GHb,INS,TC,TG,LDL-C,ALT,AST,IL-1β,IL-6,TNF-α,MDA;improve insulin resistance and oxidative stress.HE staining of liver tissue showed that Sancai Lianmei Particle could alleviate the vacuolar degeneration of liver and deposition of lipid droplets.Sancai Lianmei Particle can effectively down-regulate the expressions of ASK1,JNK and NF-κB mRNA in liver tissues of model rats.Western Blot results exhibited that Sancai Lianmei Particle could significantly regulate the expression of apoptotic proteins of Bax,Caspase-3,and Bcl-2 and inhibit the apoptosis of hepatocytes.Conclusion This study proved that Sancai Lianmei Particle can improve hepatic insulin resistance and oxidative stress,slow the progression of NAFLD by regulating liver cell apoptosis based on ROS-ASK1-JNK/NF-κB pathway.
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ObjectiveTo investigate the impact of different infusion methods on the incidence of Cinobufagin-induced phlebitis. Methods80 patients required Cinobufagin infusion were enrolled and were randomized into the observation group and the control group with 40 patients in each group.In the observation group,low dose of phentolamine was added into the Cinobufagin solution.Normal saline was infused into patients before,during and after the course of the Cinobufagin infusion.For the control group,low dose of phentolamine was added into the Cinobufagin solution without normal saline infusion.The incidence of phlebitis was compared between two groups. ResultsThe incidence of phlebitis in the observation group was 27.5％,and the control group was 60.0％.The difference was statistically significant. ConclusionsPhlebitis induced by Cinobufagin-infusion can be prevented by addition of low dose of phentolamine and infusion of normal saline solution before,during and after the course of Cinobnfagin infusion.
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AIM:To compare the effects of different histogram enhancement algorithms on improving the quality of MRI image.METHODS:Three processing algorithms,including histogram equalization,adaptive histogram equalization and histogram specification,were applied to enhance a MRI cervical spine T2-weighted image based on programming software interactive data language.The capability of representation of details in dark area and the level of noise were evaluated by means of peak signal to noise ratio and image information entropy.RESULTS:Histogram equalization cannot enhance the details in dark region obviously,but decline the contrast of whole image;adaptive histogram equalization can improve details but enlarge noise and engender shadow at edges simultaneously;histogram specification can choose the type of histogram function to match;it reveals the details in dark area sufficiently,and there is the lowest level of noise among these three algorithms.CONCLUSION:MRI cervical spine T2-weighted image processing with different algorithms of histogram enhancement,histogram specification is more outstanding than histogram equalization and adaptive histogram equalization in the representation of details and the low-level of noise.