RESUMO
In the post-genomic era, biological studies are characterized by the rapid development and wide application of a series of "omics" technologies, including genomics, proteomics, metabolomics, transcriptomics, lipidomics, cytomics, metallomics, ionomics, interactomics, and phenomics. These "omics" are often based on global analyses of biological samples using high through-put analytical approaches and bioinformatics and may provide new insights into biological phenomena. In this paper, the development and advances in these omics made in the past decades are reviewed, especially genomics, transcriptomics, proteomics and metabolomics; the applications of omics technologies in pharmaceutical research are then summarized in the fields of drug target discovery, toxicity evaluation, personalized medicine, and traditional Chinese medicine; and finally, the limitations of omics are discussed, along with the future challenges associated with the multi-omics data processing, dynamics omics analysis, and analytical approaches, as well as amenable solutions and future prospects.
Assuntos
Pesquisa Biomédica , Métodos , Perfilação da Expressão Gênica , Genômica , Metabolômica , Farmacologia , ProteômicaRESUMO
<p><b>OBJECTIVE</b>To develop an approach to the determination of saponins in Radix Cynanchi Atrati, and to optimize the parameters for purified the preparation of total saponins by macroporous resin column chromatography.</p><p><b>METHOD</b>Using cynanversicoside A as a reference, the determination of saponins was performed; according to the elution rate and the purity of the products, the preparation performance of total saponins by macroporous resin was investigated, and its parameters were optimized.</p><p><b>RESULT</b>The saponins in Radix Cynanchi Atrati were successfully determined at 518 nm by vanillin-perchloric acid as spray reagent. The macroporous resin HP-20 showed static absorption ratio of 59. 3 mg x g(-1); the 70% ethanol extraction of Radix Cynanchi Atrati was eluted from column of macroporous resin HP-20 by water and 30% ethanol, and the saponins were concentrated in 90% ethanol solution. The content of saponin part eluted from HP-20 column was 77.62%.</p><p><b>CONCLUSION</b>The proposed approach allows convenient and efficient preparation and purification of saponin in Radix Cynanchi Atrati.</p>
Assuntos
Absorção , Benzaldeídos , Química , Calibragem , Cynanchum , Química , Etanol , Química , Percloratos , Química , Porosidade , Reprodutibilidade dos Testes , Resinas Vegetais , Química , Saponinas , Química , Sensibilidade e EspecificidadeRESUMO
Metabonomics, as a newly developed technique, is expected to be a powerful technique in clinical diagnosis. Metabonomics-based diagnosis involves the global metabolic analysis of body-fluids, determination of biomarkers by multivariate statistic analysis, and establishemen of mathematic models for clinical diagnosis with the aid of pattern recognition. This article reviews the adoption of various analytical and computational strategies, application of metabonomics in clinical diagnosis, and potential challenges and development trends.
Assuntos
Humanos , Biomarcadores , Metabolismo , Líquidos Corporais , Metabolismo , Técnicas e Procedimentos Diagnósticos , Metaboloma , Metabolômica , Métodos , Modelos Teóricos , Análise MultivariadaRESUMO
<p><b>AIM</b>To evaluate the quality of Qingkailing injections effectively, and to develop a credible method for the quality control of traditional Chinese medicine.</p><p><b>METHODS</b>Fingerprints of 18 Qingkailing injection samples from various manufacturers were obtained by HPLC/ELSD, and quality evaluation was performed by constellation graphical clustering method.</p><p><b>RESULTS</b>HPLC/ELSD and constellation graphical clustering method properly revealed the quality information apparently and accurately.</p><p><b>CONCLUSION</b>It was an apparent, credible and efficient method for quality evaluation of Chinese medicines.</p>