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Objective::To study the correlation between endogenous hormone content, related enzyme activity and embryogenesis during the stratification of Acanthopanax senticosus seeds, and to provide theoretical basis for application of endogenous hormones and related physiological indicators in regulating germination of A. senticosus seeds in production and scientific research. Method::Endophytic fungi as well as different concentrations of nitric oxide and hydrogen peroxide solution were used for soaking the seeds of A. senticosus, and then thermophilic stratification was conducted for the seeds. The content of endogenous hormones such as gibberellin (GA3), abscisic acid (ABA), indole acetic acid (IAA), indole butyric acid (IBA) and salicylic acid (SA) in seeds of A. senticosus were tested by high performance liquid chromatography (HPLC), and the activity change of enzymes in vivo such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and malondialdehyde (MDA) were tested. The correlation between the embryo rate and the hormones and their enzyme activities in the seeds of A. senticosus was studied by the grey correlation method. Result::The embryo rate was significantly positively correlated with IAA, GA3, and CAT, with correlation coefficient of 1.086, 0.935 and 1.067, respectively. There was a significant positive correlation between IAA, GA3, CAT, IBA, and SA, but with a significant negative correlation with ABA, MDA, and POD. The correlation degree was as follows: IAA>CAT>GA3>IBA>SA>SOD>ABA>POD>MDA. Conclusion::IAA, GA3 and CAT have significant promoting effects on embryo rate, and there is a significant correlation between hormone content and enzyme activity, which provides a basis for exploring the seed germination mechanism of A. senticosus.
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Objective: To study the quality of Acanthopanax senticosus seedlings,develop grading standards and optimize the best analytical method. Method: Observing and measuring the plant height,diameter,leaf area,root length,chlorophyll content and other main agronomic traits of A. senticosus seedlings from different habitats(Baoqing,Qitaihe,Dongfanghong,Yilan,Acheng,Raohe Linkou and Yabuli) in each year. K-cluster grading method,principal component evaluation factor K-cluster analysis method,standard deviation method for grading,three classification methods were evaluated with different levels of seedling survival rate as indicators. Result: The direct K-cluster analysis method was used to determine the quality of A. senticosus seedlings as the best method. The seedlings of A. senticosus were divided into 3 grades,among in the first level the seedling height is ≥ 13 cm,the stem diameter is ≥ 0.37 cm,the root length is ≥ 8 cm,the leaf area is ≥ 28 cm2,the chlorophyll content is ≤ 31,the main origin is Baoqing,Qitaihe area. In the second level the seedling height is 8-13 cm,the stem diameter is 0.30-0.37 cm,the root length is 6-8 cm,the leaf area is 13-28 cm2,the chlorophyll content is 31-32,and the main origin is Acheng,Dongfanghong,Raohe area. In the third level the seedling height is 5-8 cm,the stem diameter is 0.26-0.30 cm,the root length is 5-6 cm,the leaf area is 5-13 cm2,the chlorophyll content is 32-38,and the main origin is Yabuli,Yilan and Linkou. Conclusion: In this experiment,the quality grading standards of A. senticosus seedlings were preliminarily established,Baoqing and Qitaihe can be used as a high-quality production area for breeding seeds in the Heilongjiang province.which provided the basis for quality evaluation of A. senticosus seedling planting and artificial cultivation.
RESUMO
Objective: To investigate the effect of exogenous nitric oxide (NO) on breaking the dormancy of Acanthopanax senticosus seeds and the changes in endogenous hormones and enzymes,and provide a basis for breaking the dormancy as well as artificial cultivation of A. senticosus seeds.Method: Different concentrations (1,5,10,20 mmol·L-1) of sodium nitroprusside (NO donor) were used to treat the A. senticosus seeds, and then thermophilic stratification was conducted. The content changes of endogenous hormones such as gibberellin (GA3), abscisic acid (ABA),indolo acetic acid (IAA),indolo butyric acid (IBA) and salicylic acid (SA) at different stratification time (0, 30, 50, 80, 100,130 d) were tested by high performance liquid chromatography (HPLC). The activity change of its in vivo enzymes[catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and malondialdehyde (MDA)] were tested by enzyme-labeled instrument.Result: In the seed germination process of A. senticosus,the contents of GA3,IAA,IBA,and SA were increased gradually,while the content of ABA was reduced gradually. The enzyme activities of POD and MDA were significantly reduced,and the enzyme activities of CAT and SOD were increased obviously. Exogenous NO could increase the seed germination rate and shorten the seed germination time. The effect of 20 mmol·L-1 sodium nitroprusside showed the most obvious effect and 10 mmol·L-1 SNP showed the weakest effect in promoting seed germination,showing an obvious "V" shape for changes.Conclusion: Sodium nitroprusside could promote the seed germination effect of A. senticosus, probably by increasing the content of hormones and enzyme in the stage of seed germination and improving the contents of endogenous NO during germination.
RESUMO
Objective: To study the fingerprints of Paeoniae Radix Rubra in different habitats, and determine the content of five chemical components (gallic acid, albiflorin, paeoniflorin, benzoic acid, and benzoyl paeoniflorin) and systematically cluster them. The relationship between origin and content was analyzed by grey correlation degree to provide reference for the quality evaluation of Paeoniae Radix Rubra. Methods: Fingerprints of Paeoniae Radix Rubra from 21 different producing areas were constructed by high performance liquid chromatography. The results were classified by principal component analysis and systematic cluster analysis. The gray correlation degree method was used to process the index components and their relative correlations were calculated. Results: HPLC fingerprints of Paeoniae Radix Rubra from 21 habitats were established, 11 common peaks were confirmed, and five of them (gallic acid, albiflorin, paeoniflorin, benzoic acid, and benzoyl paeoniflorin) were identified. The similarity of Paeonia lactiflora was greater than 0.9, and the similarity of Paeonia veitchii was less than 0.9. It was divided into two categories by principal component analysis combined with cluster analysis. The results of grey correlation analysis showed that the relative correlation (ri) was the largest in Gansu, followed by Ganzi in Sichuan. Conclusion: There is a big difference in the relative yield of Paeoniae Radix Rubra in different habitats. This experiment provides a scientific basis for the quality evaluation of Paeoniae Radix Rubra by fingerprint analysis, principal component analysis combined system cluster analysis and grey correlation analysis method.