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Objective To observe the effect of Jiaotai Pills ( JP) on hypothalamic neurotransmitters of Orexin A and gamma-aminobutyric acid ( GABA) in rapid eye movement ( REM) sleep deprivation rats. Methods Rat model of REM-sleep deprivation was established by water small platform method. The rats were randomized into 6 groups, namely normal control group, model group, Diazepam group (3 mg/kg), and high-, medium-and low-dose JP groups ( JP in the dosage of 18.6, 9.3 and 4.6 g/kg respectively) . Enzyme-labeled instrument was used to detect the absorbance ratio of rat hypothalamic Orexin A content, and high performance liquid phase electrochemical detection method was adopted for the detection of hypothalamic GABA content. Results Compared with the normal control group, all of the rats in the model group suffered from insomnia, and the Orexin A content was increased ( P0.05) . Conclusion The sedative and hypnic mechanism of JP is probably related with the inhibition of hypothalamic Orexin A.
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Objective To discuss the sedative and hypnotic effect of Jiaotai Pills on insomnia in PCPA rat model .Methods The PCPA ( PCPA acid) rats insomnia model was established, and the rats were divided into the model group, high, middle and low dosage of Jiaotai Pills group, and diazepam positive control group.The high performance liquid detective method was adopted to observe the effect of Jiaotai Pills on rat insomnia and hypothalamus neurotransmitters 5-HT and GABA.Results Compared with the model group, there existed significant difference in the insomnia state of rats and the central neurotransmitters 5 -HT in the high and middle dosages of Jiaotai Pill group and diazepam group.However, in the comparison of the high and middle dosages of Jiaotai Pill group and the model group, there was no significant difference in GABA in the central nervous system .Conclusion Jiaotai Pills has sedative and hypnotic efficacy.Its mechanism may be related to the effects of central neurotransmitter 5 -HT.Its relationship with GABA in the central nervous system is not obvious.The research has significant influence on the further development of Jiaotai Pills.
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Chinese medical records recorded the whole activities during a syndrome differentiation and treatment of disease. With the characteristics of diversity and lively, the records have important values in establishing TCM therapeutic thoughts and abilities for students. Amount of medical records have been recorded in medical books since the Western Han dynasty. In order to provide the references for teaching prescription aided by medical records, this article discusses the choice and application of medical records to teach prescription by listing examples on the basis of outlining the development of TCM medical records.
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Objective To explore the therapeutic mechanism of Jiaotai Pill(JP) for restoring normal coordination between the heart and the kidney.Methods Fifty SD rats were equally randomized into normal group,model group,Huanglian(Rhizoma Coptidis,15g) group,Rougui(Cortex Cinnamomi,1.5g) group,and JP group(the ratio of Huanglian and Ruigui being 15∶1.5).Insomnia rat models were induced by intraperitoneal injection of one-dose para-chloroamphetamine acid(PCPA,300mg/kg),and the serum interleukin-1(IL-1) and tumor necrosis factor(TNF-?) levels were detected by enzyme-linked immunosorbent assay(ELISA).Results In the model group,the serum levels of IL-1 and TNF-? were decreased(P0.05 compared with the model group),and increased in JP group(P
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【Objective】To compare aristolochic acid(AA)content in Caulis Aristolochiae Manshuriensis(CAM)and in the separated prescriptions of Longdan Xiegan Decoction(LXD),and to explore the effect of Chinese herbal medicine compatibility on AA content.【Methods】According to the herbs percentage compared to LXD,the herbs samples were named after: CAM group,LXD group,CAM group with heat-clearing herbs,CAM group with yin-nourishing herbs,CAM group with herbs for promoting urination,CAM group with Radix Glycyrrhizae,and LXD group with CAM removed.The AA content was determined by reverse phase HPLC.The chromatographic conditions were as follows: C18 Diamonsiltm analytical column,gradient eluted with a mixture of methyl alcohol,water and acetic acid(volume proportion as 74:24:1),and detection wavelength at 315 nm.【Results】AA content was the lowest in LXD group,and was lower in CAM group with yin-nourishing herbs than that in CAM group.【Conclusion】Subjecting to the theory of Chinese herbal medicine compatibility,it is feasible to reduce the CAM toxicity by composing a prescription with proper proportion of herbs.
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【Objective】To observe the effect of Ma Xing Shi Gan Decoction(MXSGD)on metabolism of oxygen free radicals in mice with bronchial asthma.【Methods】Forty BALB/c mice were randomized into 4 groups:normal group,model group,low-dose MXSGD group(20?g?kg~(-1)?d~(-1))and high-dose MXSGD group (80?g?kg~(-1)?d~(-1)).Except the normal group,the mice in other 3 groups received sensitization by intraperitoneal injection of ovalbumin(OVA)and challenge by inhalation of OVA to induce bronchial asthma.Meanwhile,MXSGD groups were given the corresponding doses according to the experimental design by gastric gavage,and normal group and model group received the same volume of normal saline for 27 days.Twelve-four hours after last challenge,blood sample was taken and homogenate of lung tissue was prepared for the detection of superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)contents in the plasma and the lung tissue.【Results】High-and low-dose MXSGD increased SOD and GSH-Px contents and decrease MDA content in the lung tissue and plasma(P
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[Objective] To observe the effect of Puji Xiaodu Decoction (PXD) on immune function in mice. [Methods] Thirty NIH mice were randomized into three groups. Group A was treated with normal saline, group B with 2.9 g/kg PXD and group C with 11.6 g/kg PXD for 7 days. After treatment, interleukin 2 (IL-2) production and natural killer cell (NK) activity in splenocytes and lymphocyte proliferation in the spleen were detected. [Results] PXD increased the NK activity and IL-2 production, and promoted the proliferation of splenic lymphocyte, the difference being significant as compared with group A ( P
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[Objective] To explore the therapeutic mechanism of modified Zhuling Decoction (MZD) in treating primary mesangial proliferative glomerulonephritis (PMPG). [Methods] Rat models of PMPG were induced by rabbit type antibody of rat thymocyte (Thy-1). Forty rats were randomized into four groups: A (normal control), B (model), C (treated with heparin) and D (treated with MZD). Biochemical indexes in blood, activities of cytokines such as interleukin-1? (IL-1?), tumor necrosis factor ? (TNF-?) and interleukin-6 (IL-6) and mRNA expression of IL-6 were observed. [Results] As compared with group A, serum contents of IL-1?, TNF-? and IL-6 were increased and mRNA expression of IL-6 was promoted in group B (P