RESUMO
AIM: To establish HPLC fingerprint for the identification of Hydrocotyle sibthorpioides and its adulterants. METHODS: The chromatographic seperation was performed on a Diamonsil C_(18) (250 mm×4.6 mm,5 μm)with a mobile phase consisting of acetonitrile-0.1% phosphoric acid, gradient eluent, at the flow rate of 1.0 mL/min. The UV detection was set at 340 nm. RESULTS: There was an apparent difference in fingerprint between Hydrocotyle sibthorpioides and its adulterants. CONCLUSION: The method is stable and reliable with a good reproducibility andprovides a reference standard for identifying Hydrocotyle sibthorpioides and its adulterants.
RESUMO
To explore the characteristics of thought of clinical syndrome differentiation and promote the curative effects in TCM.When treating the patients,doctors must attach great importance to the activities of qi.Only the functional activity of qi theory is studied with the combination of the holistic concept can the curative effects be improved.Perpetual motion theory can not work well without the guidance of holism and visceral theory.The theory of "treatments are determined by the variations of symptoms" which bases itself on the precondition that pathology changes in advance,constitutes one of the treatment methods under the guidance of perpetual motion theory.The word `root' in the theory of "treatment aiming at the root cause of disease" mainly refers to pathogenesis,nature and location of disease,and disease should be analyzed with pathological changes of human body to seek out the root cause of the disease.To accumulate and explore into the treatment by differentiation of syndromes will be conducive to the treatment of the patients who spend long time to cure.The essence of prescription lies in its differentiation in treatment.With the Zhongjing's spirit of prescriptions in mind,though not prescribing in the name of Zhongjing,we can call this kind of prescription is Zhongjing style.Medicine is valued in its quality and character,and also in its wise use under the guidance of TCM theories.
RESUMO
AIM: To establish HPLC fingerprint for the identification of Hydrocotyle sibthorpioides and its adulterants. METHODS: The chromatographic seperation was performed on a Diamonsil C_18(250 mm?4.6 mm,5 ?m)with a mobile phase consisting of acetonitrile-0.1% phosphoric acid,gradient eluent,at the flow rate of 1.0 mL/min.The UV detection was set at 340 nm. RESULTS: There was an apparent difference in fingerprint between Hydrocotyle sibthorpioides and its adulterants. CONCLUSION: The method is stable and reliable with a good reproducibility and provides a reference standard for identifying Hydrocotyle sibthorpioides and its adulterants.
RESUMO
AIM:To establish HPLC fingerprint for the root and rhizome of Clematis uncinata Champ and to compare the differences of clematis and Clematis uncinata in fingerprint. METHODS:Based on 10 batches of Clematis unciuata Champ,its chromatographic seperation was performed on Diamonsil C18 (250 mm ? 4. 6 mm,5 ?m)with a mobile phase consisting of acetonitrile -0.05% phosphoric acid,gradient eluent,at the flow rate of 0. 8 mL/min. The UV detection was set at 210 nm. RESULTS:The mutual mode to HPLC-UV fingerprints was set up,and the 23 mutual peaks were indicated. The similarities were compared among Cleuatis uncinata Champ and substitutes collected from different sources there were apparent difference in fingerprint. CONCLUSION:The method is stable and reliable with a good reproducibility and provides a reference standard for identifying medicinal clematis.