RESUMO
Digestive enzymes and antioxidants present in food materials act as a surplus for our digestive system. In present study, five digestive enzymes namely amylase, protease, lipase, pectinase and α -glucosidase and seven antioxidant assays for Catalase, Peroxidase, H2O2, Super Oxide Dismutase, Malondialdehyde, Carotenoid & radical scavenging were carried out from two members of cucurbitaceae family namely Cucurbita pepo (Pumpkin) and Langenaria siceraria (bottle gourd). Amongst them amylase, lipase and pectinase activity were found in C. pepo and L. siceraria. The enzyme kinetics studies revealed their maximum velocities to be 0.2631 and 0.33557 mM min-1 for Amylase and Lipase respectively. Our further studies report the presence of proteinase inhibitors and α-gluosidase inducers, while absence of amylase inhibitors and α -glucosidase inhibitors in pumpkin and bottle gourd. Present findings suggest the use of these fruits as an attractive material for further study leading to possible development of digestive syrup.