Studies on the prolongation of cardiac allograft survival by alloreactive lymphocytes depletion. indaection of allograft immunotolerance by alloreactive lymphocyte vaccine in recipient preparation / 临床外科杂志

Objective Alloreactive lymphocytes specific for graft antigens are implicated in rejection of allograft.This study aims to induce alloreactive lymphocytes depletion or inactivation and allograft immuno tolerance by alloreactive lymphocyte vaccine(ALV).Methods Recipient lymphocytes were expanded for 9-14 days stimulated by donor lymphocytes that had been dealt with mitomycin C.The expanded recipient lymphocytes are termed alloreactive lymphocyte vaccine(ALV).About 0.5?10 7～1.0?10 7cells mixed with freunds complete ajuvant(FCA)were used as ALV to vaccinate itself for 3～4 times as usual. The stimulate index(SI) was measured after vaccination and compared with itself unvaccination. Results The ALV could suppress mixed lymphocyte reaction. The stimulate Index was significantly lower than themselves uninjected with ALV(P