A study on the targeted nanoparticles of isosorbide mononitrate on reducing the levels of inflammatory factors in rabbit models of rhinosinusitis / 中华耳鼻咽喉头颈外科杂志

Objective@#To investigate the effect of isosorbide mononitrate (ISMN) targeted nanoparticles on inflammatory factors of sinusitis by establishing a rabbit model of rhinosinusitis.@*Methods@#Thirty healthy rabbits, male and female, weighing 2.5-3.5 kg, were randomly divided into 6 groups with 5 rabbits in each group. Group A was the control group. The model of rabbit sinusitis was established in group B to F, and CT was used to confirm the model was successful. After placing tubes into the maxillary sinus in the group C to F, saline, 45 mg/ml ISMN, 45 mg/ml ISMN nanoparticles and 45 mg/ml ISMN targeted nanoparticles were used to wash the maxillary sinus, respectively. Blood samples were collected from the ear vein of rabbits on day 7, 14, 21, 28, 35 and 42 after modeling respectively. Changes in the expression levels of inflammatory factors in rabbits during the modeling process and after drug washing were detected by ELISA. After the drug intervention, the maxillary sinus mucosa was taken for real-time quantitative PCR to detect the changes in the mRNA level of inflammatory factors. SPSS 22.0 software was used to process the data.@*Results@#Rabbit model of sinusitis was successfully established. ELISA showed that after the action of ISMN targeted nanoparticles of 1 week (42th day after modeling), the levels of (interleukin, IL) 4, IL-8, IL-17A and interferon γ (IFN-γ) in the blood were lower compared with that of 35th day after modeling, the difference was statistically significant (5.57±1.20 vs 19.73±0.68, 66.41±11.87 vs 154.68±13.13, 17.96±1.87 vs 28.23±0.80, 53.56±5.66 vs 111.93±7.29, all P<0.05). Compared with the ISMN nanoparticles and ISMN, the ISMN targeted nanoparticles reduced the levels of IL-4, IL-8, IL-17A and IFN-γ more obviously, the differences were statistically significant (13.26±1.43 vs 8.81±1.33 vs 7.14±2.16, 89.47±17.80 vs 41.07±7.77 vs 15.84±3.72, 10.28±2.07 vs 3.06±1.62 vs 1.82±0.90, 62.16±6.18 vs 35.12±4.62 vs 27.89±10.18, all P<0.05). Real-Time PCR showed that after the flushing of ISMN targeted nanoparticles, the levels of IL-4, IL-8, IL-17A and IFN-γ mRNA were lowest compared with that of the model group, ISMN nanoparticles and ISMN group.@*Conclusion@#ISMN targeted nanoparticles can reduce the level of inflammatory factors in rabbit sinusitis model.

Therapeutic effect of sunflower powder active ingredients on hyperuricemia in mice / 吉林大学学报(医学版)

Objective:To investigate the therapeutic effects of small peptide,alkaloid,total flavonoids and polysaccharides extracted from sunflower plate powder on the hyperuricemia in the mice,and to clarify that the influence of sunflower powder in the uric acid(UA)level and joint swelling and its protective effect on the liver and kidney tissues.Methods:A total of 96 male Kunming rats were randomly divided into 8 groups,each group of 12 rats.One group was used as blank control group,the others were used to set up hyperuricemia models.The successful mouse models of hyperuricemia were randomly divided into model group and positive control group (allopurinol group),small molecule peptide group,alkaloi group,total flavone group,polysaccharide group and sunflower disk group.The serum UA levels were measured 7 d after administration and the liver and kidney tissues of the mice in various groups were taken.The morphological changes of liver and kidney tissues were observed by HE staining.Acute gouty arthritis model was established by injecting 3 mg sodium urate into the knee joint of the male SD rats.The experiment was divided into blank group,model group,positive control colchicine group, sunflower disc extracts(SDE)group with different concentrations(0%,20%,40%,60%,80% and 100%), 4 rats in each group.The joint circumference was measured at 0,12,24 and 48 h,respectively.The serum levels of interleukin-10(IL-10)and macrophage inflammatory protein-1α(MIP-1α)were detected by ELISA kit. Results:Compared with model group,the serum UA levels of the mice in allopurinol group,small molecule peptide group,alkaloid group,total flavonoids group and sunflower group were significantly decreased after administration (P<0.05);and the serum UA level in allopurinol group was decreased the most,followed by small molecule peptide group.The HE staining results showed that the hepatocytes in small molecule peptide group were clear, the hepatocytes had full cytoplasm,and no abnormality was observed in the kidney cells compared with blank control group.After 48 h injection of sodium urate,the degree of joint swelling was reduced by up to 27.1% in SDE group compared with model group.Compared with model group,the serum IL-10 level in 20% SDE group was significantly increased(P<0.01),and the serum MIP-1α level was also significantly increased(P<0.01). Conclusion:Sunflower powder can reduce the UA level and has protective effect on the liver and kidney tissues and it can improve the body's anti-inflammatory activity and reduce the body's inflammatory response to achieve anti-gout arthritis activity.

Analysis on therapeutic efficacy of different approaches for treating fungal maxillary sinusitis / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To compare the response of simple endoscopic surgery and endoscopic surgery plus small windows through canine fossa surgery for fungal maxillary sinusitis.@*METHOD@#Twenty-five patients were applied the endoscopic surgery plus small windows through Canine fossa surgery as the treatment group, and the other 25 were applied the simple endoscopic surgery as control.@*RESULT@#During a follow up for 1-2 years, the effect of fungal maxillary sinusitis under endoscopic by dual approach is superior to simple endoscopic. There were obvious differences between the two groups(chi2 = 4.268, P < 0.05).@*CONCLUSION@#Endoscopic surgery plus small windows through canine fossa surgery for fungal maxillary sinusitis can fully expose maxillary sinus cavity,destroy the abnormal tissues completely, change the low oxygen environment, ensure the normal physiological function of maxillary sinus mucosa, and really achieve radical minimally invasive purpose.

Challenges facing hospitals in the new century and development strategies / 中华医院管理杂志

The authors explained in the first place the challenges facing hospitals in the new century. These include: impact of the reform of the social security system on hospitals; impact of China's accession to the WTO on domestic hospitals; and impact of people's diversified needs on the construction of hospitals. Then the authors set forth some development strategies for hospitals in the new century. These include: ①establishment of new concepts; ②reinforcement of awareness in four aspects, viz. management awareness, competition awareness, service awareness, and innovation awareness; ③stress on the systematic nature of hospital construction, viz. market-and battlefield-oriented adjustment of the layout of disciplines, construction of the contingent of talents in accordance with the needs of the market, and enhancement of medical quality in light of new quality concepts; ④establishment of a flexible and highly efficient management mechanism, viz. setting up a speedy and highly efficient decision-making procedure and an individual-centered management mechanism, reforming the distribution mechanism in line with the rules of the market economy, and finding ways for the government to solve the problem of funds compensation for non-profit hospitals.

Stressing the cultivation and supervision of professional cadres with high academic degrees / 中华医院管理杂志

Having its long-term development in mind, our hospital has attached great importance to the cultivation of personnel with high academic degrees and marked effects have been achieved in this aspect. Major ways adopted include: ①improvement in an all-round way of the overall quality of personnel with high academic degrees, with emphasis put on the enhancement of their clinical skills through practice, enhancement of their research capabilities, and cultivation of a sense of independence and innovative abilities; ②instillation of three senses in personnel with high academic degrees, viz. sense of dedication, sense of orienting medical service towards the needs of patients, and sense of dynamic enterprise; ③optimization of the environment so as to encourage personnel with high academic degrees to display their abilities; ④dynamic supervision of personnel with high academic degrees through selecting the superior and eliminating the inferior.