Non-C21 steroids from the Rhizome of Cynanchum stauntonii / 中国药科大学学报

@#Twelve compounds were isolated and purified from ethyl acetatefraction of Cynanchum stauntonii by silica gel, Sephadex LH-20 and ODS column chromatography. Their structures were identified by spectral techniques and physicochemical properties as syringaresinol(1), (-)-(7R, 7′R, 7″R, 8S, 8′S, 8″S)-4′, 4″-dihydroxy-3, 3′, 3″, 5-tetramethoxy-7, 9′ ∶7′, 9-diepoxy-4, 8″-oxy-8, 8′-sesquineolignan-7″, 9″-diol(2), prinsepiol(3), 4-hydroxyacetophenone(4), baishouwubenzophenone(5), 2, 4-dihydroxyacetophenone(6), benzoic acid(7), 1, 4-benzenediol(8), 6-O-［E］-sinapoyl-α-D-glucopyranoside(9a), 6-O-［E］-sinapoyl-β-D-glucopyranoside(9b), 1-O-methyl-α-D-cymadropyranoside(10), β-daucosterol(11), and β-sitosterol(12). Compounds 1-3, 5 and 7-11 were firstly isolated from this plant.

Chemical constituents of Fructus Gleditsiae Abnormalis / 中国药科大学学报

@#Twelve compounds were isolated from the ethanol extract of Fructus Gleditsiae Abnormalis by macroporous resin, silica gel, Sephadex LH-20, MCI and ODS column chromatographies. Their structures were identified on the basis of physicochemical properties and spectral data as gleditsioside A(1), gleditsioside B(2), gleditsioside H(3), gleditsioside I(4), gleditsioside J(5), gleditsioside K(6), gleditsia saponins C′(7), tamarixetin-7-O-β-D-glucopyranoside(8), neohesperidin(9), chrysoeirol-7-O-neohesperidoside(10); syringaresinol- O-β-D-glucopyranoside(11), liriodendrin(12). Compounds 8-12 were firstly isolated from this genus.

Chemical constituents from the leaves of Callicarpa nudiflora / 中国药科大学学报

From the n-butanol extract of the leaves of Callicarpa nudiflora,thirteen compounds were isolated by repeated column chromatography with silica gel,C_(18) and Sephadex LH-20.Their structures were identified by spectroscopic (~1H,~(13)C NMR and so on) and chemical methods as luteolin (1),luteolin-7-O-β-D-glucopyran coside (2),apigenin (3),5,7,4'-trihydroxy-3'-methoxyflavone (4),luteolin-3'-O-β-D-glucopyranoside (5),api genin-7-O-β-D-glucopyranoside (6),luteolin-7-O-(6-trans-caffeoyl)-β-D-glucopyranoside (7),luteolin-7-O-(6-trans-feruloyl)-β-D-glucopyranoside (8),arjunglucoside I(9),luteolin-7-O-(6-p-coumaryl)-β-D-glucopyranoside (10),forsythoside B (11),isomartynoside (12),deacylisomartynoside B (13).Among them,compound 11 was isolated from this plant for the first time and compounds 4,7-10,12-13 were isolated from this genus for the first time.

CYCLOARTANE TRITERPENES AND GLYCOSIDES FROM CIMICIFUGA ACERINA / 药学学报

AIM　To investigate the chemical constituents from the rhizomes of Cimicifuga acerina (Sieb. et Zucc.) Tanaka. METHODS　Column chromatgraphy (including silica gel and ODS) together with HPLC was used to separate the chemical constituents whose structures were determined by FAB-MS, NMR (1D and 2D) and hydrolysis methods. RESULTS　Five cycloartane triterpenoids were isolated and identified as: (22R)-22-hydroxycimigenol (I), (22R)-22-hydroxy-24-O-acetylhydroshengmanol 3-O-β-D-xylopyranoside (II), dahurinol (III), 24-epi-24-O-acetyl-7,8-didehydroshengmanol 3-O-β-D-xylopyranoside (IV), 25-O-acetyl-7,8-didehydrocimigenol 3-O-β-D-xylopyranoside (V). CONCLUSION　Compound I is a new natural product, compound II is a new compound and compounds IV and V were isolated from this plant for the first time.

Minor Phenolic Constituents of Chinaberry-tree (Melia azedarach) / 中草药

Five minor ferulic acid esters (Ⅰ～Ⅴ) were identified in a petrol extract of Melia azedarach stem bark, one (Ⅰ) of which was isolated with no impurity for the first time. By chemical and spectral methods, their structures were elucidated as hexacosylferulate (Ⅰ); tetracosylferulate (Ⅱ); pentacosylferulate (Ⅲ); heptacosylferulate (Ⅳ) and octacosylferulate (Ⅴ), respectively.

A new coumarin glycoside from Bombyx Batryticatus / 中草药

Object To study and identify the chemical constituents of Bombyx Batryticatus. Methods The constituents were isolated from the above materials by column chromatography using silica gel and Sephadex LH-20, purified by crystallization, and identified by spectroscopic methods. Results Eight compounds were isolated and identified. They are 6-methoxy-7-O-?-D-(4′-methoxy) glucopyranosyl coumarin (Ⅰ), ergost-6, 22-dien-3?, 5?, 8?-triol (Ⅱ), palmitic acid (Ⅲ), meso-erythritol (Ⅳ), D-mannitol (Ⅴ), uracil (Ⅵ), ?-sitosterol (Ⅶ), and daucosterol (Ⅷ). Conclusion Coumpound Ⅰ is a new coumarin glycoside.

Studies on chemical constituents of Cimicifuga dahurica / 中草药

Object To isolate and identify the chemical constituents from the rhizome of Cimicifuga dahurica (Turcz ) Maxim Methods The different chromatographic techniques were used to isolate ten constituents, and the spectral methods, such as IR, MS, 1HNMR, 13 CNMR and 2DNMR, were used to identify the structures Results Their structures were identified as cimigenol (Ⅰ), 24 epi 7, 8 didehydrocimigenol 3 O ? D xylopyranoside (Ⅱ), 7, 8 didehydrocimigenol 3 O ? D xylopyranoside (Ⅲ), 25 O acetyl 7, 8 didehydrocimigenol 3 O ? D xylopyranoside (Ⅳ), 3 arabinosyl 24 O acetylhydroshengmanol 15 glucoside (Ⅴ), isoferulic acid (Ⅵ), (E) 3 (3′ methyl 2′ butenylidene) 2 indolinone (Ⅶ), sucrose (Ⅷ), ? sitosterol (Ⅸ) and stigmastenol 3 O ? D glucopyranoside (Ⅹ), respectively Conclusion Compounds Ⅱ Ⅳ, Ⅹ are isolated for the first time from the title plant