RESUMO
The development of radioprotective agents has been the subject of intense research in view of their potential for use within a radiation environment, such as space exploration, radiotherapy and even nuclear war. However, no ideal synthetic radioprotectors are available at present, so the search for alternative sources, including plants, has been on going for several decades. This article reviews some of the most promising plants, and their radioprotection related activities.
Assuntos
Animais , Humanos , Medicamentos de Ervas Chinesas , Usos Terapêuticos , Fitoterapia , Métodos , Plantas Medicinais , Química , Lesões por Radiação , Tratamento Farmacológico , Protetores contra Radiação , Usos TerapêuticosRESUMO
<p><b>OBJECTIVE</b>To clone the influenza A virus NP gene into expression vector and to purify the target protein, which was used to study the preparation of monoclonal antibody.</p><p><b>METHODS</b>The RNA of influenza A virus was extracted and primers were designed according the NP gene sequence, then the NP gene of influenza A virus was expressed in E.coli DH5alpha and the NP protein was purified by affinity chromatography.</p><p><b>RESULTS</b>The recombinant expression vector-pGEX-4T-2-NP was successfully constructed and relatively pure target protein was obtained.</p><p><b>CONCLUSION</b>Through reasonably controlling the fermentation time, growth temperature and induction concentration, satisfactory soluble target product was obtained.</p>