Multilocus sequence typing scheme of Pseudomonas aeruginosa isolates from naval divers / 第二军医大学学报

Objective: To investigate the genetic phenotypes of Pseudomonas aeruginosa isolates from naval divers, so as to obtain the epidemic character of Pseudomonas aeruginosa isolates in different naval divers. Methods: A total of 64 Pseudomonas aeruginosa strains from naval divers were sequenced by multilocus sequence typing (MLST) using 7 housekeeping genes: acsA, aroE, guaA, muth, nuoD, ppsA and trpE. The sequencing results were analyzed based on Pseudomonas aeruginosa international MLST database to obtain the allelic profiles and sequence types (STs). MLST data were analyzed by Bionumerics 4. 0 (http-J/pubmlst. org/mlstanalyse) using LIAN, SplitsTree and eBURST. Results: Data from MLST revealed a high diversity among the strains. Out of the 64 strains, 53 strains could be assigned to 19 STs and 11 could not be assigned. ST274 and ST260 were the dominant strains, with ST274 accounting for 18. 75% (12/64) and ST260 accounting for 15. 62% (10/64). Conclusion: Pseudomonas aeruginosa strains in naval divers have high diversity and preponderant genotypes. MLST is of great significance for Pseudomonas aeruginosa genomic study and epidemiology surveillance.

The effects of cell growth factors on proliferation and migration of coronary artery endothelial cells / 中国应用生理学杂志

<p><b>AIM</b>To study the effects of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) on proliferation and migration of bovine coronary artery endothelial cells (BCAEC) in vitro.</p><p><b>METHODS</b>BCAECs were isolated and cultured in vitro, and divided into control group, VEGF group and HGF group. BCACEs proliferation were measured using MTT, and their migration was observed using reverse microscope.</p><p><b>RESULTS</b>The OD value of control, VEGF and HGF group were 0.22 +/- 0.01, 0.40 +/- 0.14, 0.44 +/- 0.15 respectively. The proliferation ratio of BCAECs in VEGF and HGF group was 81.8% +/- 16.9%, 100.0% +/- 21.1% respectively. There was no migration in control group, but significant migration in VEGF and HGF group.</p><p><b>CONCLUSION</b>Both VEGF and HGF can promote proliferation and migration of BCAECs, the effect of HGF is stronger than VEGF.</p>