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1.
Chinese Journal of Biotechnology ; (12): 2623-2632, 2021.
Artigo em Chinês | WPRIM | ID: wpr-887828

RESUMO

α-L-rhamnosidase is a very important industrial enzyme that is widely distributed in a variety of organisms. α-L-rhamnosidase of different origins show functional diversity. For example, the optimal pH of α-L-rhamnosidase from bacteria is close to neutral or alkaline, while the optimal pH of α-L-rhamnosidase from fungi is in the acidic range. Furthermore, the enzymatic properties of α-L-rhamnosidases of different origins differ in terms of the optimal temperature, the thermal stability, and the substrate specificity, which determine the different applications of these enzymes. In this connection, it is crucial to elucidate the similarities and differences in the catalytic mechanism and substrate specificity of α-L-rhamnosidase of different origins through analyzing its enzymatic properties. Moreover, it is important to explore and understand the effects of aglycon and metal cations on enzyme activity and the competitive inhibition of L-rhamnose and glucose on enzymes. These knowledge can help discover α-L-rhamnosidase of industrial significance and promote its industrial application.


Assuntos
Glicosídeo Hidrolases/metabolismo , Concentração de Íons de Hidrogênio , Ramnose , Especificidade por Substrato , Temperatura
2.
Chinese Journal of Biotechnology ; (12): 781-788, 2012.
Artigo em Chinês | WPRIM | ID: wpr-342442

RESUMO

As an efficient and promising protein engineering strategy, directed evolution includes the construction of mutant libraries and screening of desirable mutants. A rapid and high-throughput screening method has played a critical role in the successful application of directed evolution strategy. We reviewed several high-throughput screening tools which have great potential to be applied in directed evolution. The development of powerful high-throughput screening tools will make great contributions to the advancement of protein engineering.


Assuntos
Evolução Molecular Direcionada , Métodos , Ensaios de Triagem em Larga Escala , Métodos , Mutagênese Sítio-Dirigida , Métodos , Proteínas Mutantes , Genética , Engenharia de Proteínas , Métodos
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